Instability and Stasis Among the Microbiome of Seagrass Leaves, Roots and Rhizomes, and Nearby Sediments Within a Natural pH Gradient

• Published in: Microbial ecology 2022-10, Vol.84 (3), p.703-716
• Main Authors: Banister, Raymond B., Schwarz, Melbert T., Fine, Maoz, Ritchie, Kim B., Muller, Erinn M.
• Format: Article
• Language: English
• Subjects: Acidification; Aquatic plants; Bacteria; Bacteria - genetics; Biodiversity; Biomedical and Life Sciences; Ecological effects; Ecology; Ecosystems; Environmental changes; Environmental conditions; Geoecology/Natural Processes; High temperature; Hot spots (geology); Hydrogen-Ion Concentration; Leaves; Life Sciences; Marine ecosystems; Microbial activity; Microbial Ecology; Microbiology; Microbiology of Aquatic Systems; Microbiomes; Microbiota; Microorganisms; Nature Conservation; Nutrient availability; Ocean acidification; pH effects; Proton-Motive Force; Rhizome - chemistry; Rhizomes; RNA, Ribosomal, 16S - analysis; RNA, Ribosomal, 16S - genetics; Roots; rRNA 16S; Sea grasses; Seawater - microbiology; Sediment; Sediments; Statistical analysis; Statistical methods; Taxonomy; Water Quality/Water Pollution
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-021-01867-9

Seagrass meadows are hotspots of biodiversity with considerable economic and ecological value. The health of seagrass ecosystems is influenced in part by the makeup and stability of their microbiome, but microbiome composition can be sensitive to environmental change such as nutrient availability, elevated temperatures, and reduced pH. The objective of the present study was to characterize the bacterial community of the leaves, bulk samples of roots and rhizomes, and proximal sediment of the seagrass species Cymodocea nodosa along the natural pH gradient of Levante Bay, Vulcano Island, Italy. The bacterial community was determined by characterizing the 16S rRNA amplicon sequencing and analyzing the operational taxonomic unit classification of bacterial DNA within samples. Statistical analyses were used to explore how life-long exposure to different pH/ p CO 2 conditions may be associated with significant differences in microbial communities, dominant bacterial classes, and microbial diversity within each plant section and sediment. The microbiome of C. nodosa significantly differed among all sample types and site-specific differences were detected within sediment and root/rhizome microbial communities, but not the leaves. These results show that C. nodosa leaves have a consistent microbial community even across a pH range of 8.15 to 6.05. The ability for C. nodosa to regulate and maintain microbial structure may indicate a semblance of resilience within these vital ecosystems under projected changes in environmental conditions such as ocean acidification.