Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems

In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry f...

Full description

Saved in:
Bibliographic Details
Published in:Chemical science (Cambridge) 2022-11, Vol.13 (42), p.12577-12587
Main Authors: Qi, Qianqian, Zhang, Yutong, Xiong, Wei, Liu, Xingyu, Cui, Shuangyu, Ye, Xiaofang, Zhang, Kaisong, Tian, Tian, Xiang Zhou
Format: Article
Language:English
Subjects:
Chemistry
Genetic modification
Ribonucleic acid
RNA
Switches
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry for controlling CRISPR systems. Moreover, the manipulation of gene editing in human cells is accomplished. Here, norbornene-tetrazine ligation chemistry is harnessed to control RNA-guided CRISPR systems in vitro and in human cells.
ISSN:2041-6520
2041-6539
DOI:10.1039/d2sc02635j