Genome-wide identification and expression analysis of the bHLH gene family in cauliflower (Brassica oleracea L.)

• Published in: Physiology and molecular biology of plants 2022-09, Vol.28 (9), p.1737-1751
• Main Authors: Jiang, Hanmin, Liu, Lili, Shan, Xiaozheng, Wen, Zhenghua, Zhang, Xiaoli, Yao, Xingwei, Niu, Guobao, Shan, Changliang, Sun, Deling
• Format: Article
• Language: English
• Subjects: Abscisic acid; Accumulation; Anthocyanins; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Brassica; Brassica oleracea; Cauliflowers; Cell Biology; Chromosomes; Collinearity; Evolution; Gene expression; Genes; Genomic analysis; Helix-loop-helix proteins (basic); Life Sciences; Network analysis; Plant growth; Plant Physiology; Plant Sciences; Plant species; Research Article; Subgroups; Transcription factors
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-022-01238-9

Basic helix-loop-helix (bHLH) transcription factors (TFs) are one of the largest TF families in plant species, and they play important roles in plant growth, development and stress responses. The present study systematically identified members of the cauliflower ( Brassica oleracea L.) bHLH gene family based on genomic data. Analysis of bHLH family gene numbers, evolution, collinearity, gene structures and motifs indicated that cauliflower contained 256 bHLH family genes distributed on 10 chromosomes. Most of these genes have been localized in the nucleus, and they were divided into 18 subgroups which have been relatively conserved during evolution. Promoter analysis showed that most cis-acting elements were related to MeJA and ABA. Expression analysis suggested that 14 bHLH genes may be involved in the transformation of cauliflower curd from white to purple. An expression analysis of these 14 genes in FQ136 material was performed using qRT–PCR, and 9 bHLH genes ( BobHLH1, 14, 58, 61, 63, 84, 231, 239 and 243 ) showed significantly increased or decreased expression in cauliflower from white to purple, which suggests that these 9 genes play important roles in the accumulation of anthocyanins in cauliflower. The coexpression network of these 9 genes and anthocyanin synthesis-related key genes was analyzed using weighted gene coexpression network analysis (WGCNA). In conclusion, our observations suggested that the bHLH gene family plays an important role in the accumulation of anthocyanins in cauliflower and provide an important theoretical basis for further research on the functions of the bHLH gene family and the molecular mechanism of cauliflower coloration.