Effect of the Graphene Nanosheet on Functions of the Spike Protein in Open and Closed States: Comparison between SARS-CoV‑2 Wild Type and the Omicron Variant

• Published in: Langmuir 2022-11, Vol.38 (45), p.13972-13982
• Main Authors: Yan, Zeng-Shuai, Li, Xiao-Lei, Ma, Yu-Qiang, Ding, Hong-Ming
• Format: Article
• Language: English
• Subjects: Angiotensin-Converting Enzyme 2; COVID-19; Graphite; Humans; Nanostructures; Peptidyl-Dipeptidase A - metabolism; Prospective Studies; Protein Binding; SARS-CoV-2; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - metabolism
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/acs.langmuir.2c02316

The spread of coronavirus disease 2019 caused by SARS-CoV-2 and its variants has become a global health crisis. Although there were many attempts to use nanomaterials-based devices to fight against SARS-CoV-2, it still remains elusive as to how the nanomaterials interact with SARS-CoV-2 and affect its biofunctions. Here, taking the graphene nanosheet (GN) as the model nanomaterial, we investigate its interaction with the spike protein in both WT and Omicron by molecular simulations. In the closed state, the GN can insert into the region between the receptor binding domain (RBD) and the N-terminal domain (NTD) in both wild type (WT) and Omicron, which keeps the RBD in the down conformation. In the open state, the GN can hamper the binding of up RBD to ACE2 in WT, but it has little impact on up RBD and, even worse, stimulates the down-to-up transition of down RBDs in Omicron. Moreover, the GN can insert in the vicinity of the fusion peptide in both WT and Omicron and prevents the detachment of S1 from the whole spike protein. The present study reveals the effect of the SARS-CoV-2 variant on the nanomaterial–spike protein interaction, which informs prospective efforts to design functional nanomaterials against SARS-CoV-2.