Evaluating the effect of LPS from periodontal pathogenic bacteria on the expression of senescence‐related genes in human dental pulp stem cells

The human dental pulp stem cells (hDPSCs) are one of the readily available sources of multipotent mesenchymal stem cells (MSCs) and can be considered as a type of tool cells for cell‐based therapies. However, the main limitation in the clinical use of these cells is DPSC senescence, which can be ind...

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Published in:Journal of cellular and molecular medicine 2022-11, Vol.26 (22), p.5647-5656
Main Authors: Sattari, Mandana, Masoudnia, Mina, Mashayekhi, Kazem, Hashemi, Seyed Mahmoud, Khannazer, Nikoo, Sattari, Sepanta, Mohammadian Haftcheshmeh, Saeed, Momtazi‐Borojeni, Amir Abbas
Format: Article
Language:English
Subjects:
Bacteria
CDKN1A
CDKN2A
Dental pulp
DPSCs
E coli
Escherichia coli
Flow cytometry
Gene expression
Lipopolysaccharides
LPS
Mesenchyme
Molecular modelling
Original
p53 Protein
Proteins
Senescence
SIRT1
SIRT1 protein
Stem cells
TP53
Tumor necrosis factor-TNF
Western blotting
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Summary:The human dental pulp stem cells (hDPSCs) are one of the readily available sources of multipotent mesenchymal stem cells (MSCs) and can be considered as a type of tool cells for cell‐based therapies. However, the main limitation in the clinical use of these cells is DPSC senescence, which can be induced by lipopolysaccharide (LPS) of oral pathogenic bacteria. Up to now, far little attention has been paid to exploring the molecular mechanisms of senescence in DPSCs. So, the current study aimed to investigate the underlying molecular mechanism of senescence in hDPSCs stimulated with Porphyromonas gingivalis (P. gingivalis) and Escherichia coli (E. coli)‐derived LPSs, by evaluating both mRNA and protein expression of four important senescence‐related genes, including TP53, CDKN1A, CDKN2A and SIRT1. To this purpose, hDPSCs were stimulated with different LPSs for 6, 24 and 48 h and then the gene expression was evaluated using quantitative real‐time polymerase chain reaction (qPCR) and western blotting. Following stimulation with P. gingivalis and E. coli‐derived LPSs, the relative mRNA and protein expression of all genes were significantly up‐regulated in a time‐dependent manner, as compared with unstimulated hDPSCs. Moreover, the hDPSCs stimulated with P. gingivalis LPS for 6 and 24 h had the highest mRNA expression of CDKN1A and SIRT1, respectively (p 
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.17594