Heat-shocked monocytes are resistant to Staphylococcus aureus-induced apoptotic DNA fragmentation due to expression of HSP72

Human peripheral blood monocytes became apoptotic following phagocytosis of Staphylococcus aureus. The consequences of heat stress for monocytes were studied with regard to the effect on S. aureus-induced apoptosis. Exposure of monocytes to 41.5 degrees C for 1 h resulted in HSP72 expression and had...

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Bibliographic Details
Published in:Infection and immunity 1999-08, Vol.67 (8), p.4216-4222
Main Authors: GUZIK, K, BZOWSKA, M, DOBRUCKI, J, PRYJMA, J
Format: Article
Language:English
Subjects:
Apoptosis
Bacteriology
Biological and medical sciences
Cells, Cultured
DNA Fragmentation
Fundamental and applied biological sciences. Psychology
Heat-Shock Proteins - physiology
Hot Temperature
HSP72 Heat-Shock Proteins
Humans
Microbiology
Molecular and Cellular Pathogenesis
Monocytes - physiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phagocytosis
Staphylococcus aureus
Staphylococcus aureus - pathogenicity
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Summary:Human peripheral blood monocytes became apoptotic following phagocytosis of Staphylococcus aureus. The consequences of heat stress for monocytes were studied with regard to the effect on S. aureus-induced apoptosis. Exposure of monocytes to 41.5 degrees C for 1 h resulted in HSP72 expression and had no influence on phagocytosis of bacteria; moreover, phagocytosis of S. aureus immediately or shortly after heat shock had no effect on the S. aureus-induced monocyte apoptosis, as evidenced by DNA fragmentation assay. In contrast, cells which recovered from heat shock for 18 to 24 h, although active as phagocytes, were resistant to the S. aureus-induced apoptosis. The observed protective effect was related to the induction of HSP72, since blocking of HSP72 synthesis by an antisense oligomer abolished the protective effect of heat shock on bacterium-induced monocyte apoptosis.
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.67.8.4216-4222.1999