Nucleus size and its effect on nucleosome stability in living cells

• Published in: Biophysical journal 2022-11, Vol.121 (21), p.4189-4204
• Main Authors: Efremov, Artem K., Hovan, Ladislav, Yan, Jie
• Format: Article
• Language: English
• Subjects: Cell Nucleus - metabolism; Cell Nucleus Size; Chromatin - metabolism; DNA - chemistry; Nucleosomes - metabolism
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/j.bpj.2022.09.019

DNA architectural proteins play a major role in organization of chromosomal DNA in living cells by packaging it into chromatin, whose spatial conformation is determined by an intricate interplay between the DNA-binding properties of architectural proteins and physical constraints applied to the DNA by a tight nuclear space. Yet, the exact effects of the nucleus size on DNA-protein interactions and chromatin structure currently remain obscure. Furthermore, there is even no clear understanding of molecular mechanisms responsible for the nucleus size regulation in living cells. To find answers to these questions, we developed a general theoretical framework based on a combination of polymer field theory and transfer-matrix calculations, which showed that the nucleus size is mainly determined by the difference between the surface tensions of the nuclear envelope and the endoplasmic reticulum membrane as well as the osmotic pressure exerted by cytosolic macromolecules on the nucleus. In addition, the model demonstrated that the cell nucleus functions as a piezoelectric element, changing its electrostatic potential in a size-dependent manner. This effect has been found to have a profound impact on stability of nucleosomes, revealing a previously unknown link between the nucleus size and chromatin structure. Overall, our study provides new insights into the molecular mechanisms responsible for regulation of the nucleus size, as well as the potential role of nuclear organization in shaping the cell response to environmental cues.