Diagnosis and successful treatment of Brazillian porcupine poxvirus infection in a free-ranging hairy dwarf porcupine (Coendu spinosus)

• Published in: Brazilian journal of microbiology 2022-12, Vol.53 (4), p.2321-2327
• Main Authors: Guerra, Juliana Mariotti, Navas-Suárez, Pedro Enrique, Ferreira-Machado, Eduardo, Ervedosa, Ticiana Brasil, Figueiredo, Ketlyn Bolsachini, de Carvalho, Ana Carolina Souza Ramos, Silva, Maraya Lincoln, Caiaffa, Mayara Grego, da Costa, André Luiz Mota, Teixeira, Rodrigo Hidalgo Friciello, Buti, Thais Eleonora Madeira, Luchs, Adriana, Namiyama, Gislene Mitsue, de Lima, Luana Barbosa, Taniwaki, Noemi Nosomi, Matsumoto, Patricia Sayuri Silvestre, de Azevedo Fernandes, Natália Coelho Couto
• Format: Article
• Language: English
• Subjects: Amplification; Animals; Biomedical and Life Sciences; Biopsy; Construction materials; Cytoplasm; Degeneration; Deoxyribonucleic acid; Dermatitis; Diagnosis; Dipyrone; DNA; Edema; Enrofloxacin; Food Microbiology; Histopathology; Inclusion bodies; Infections; Lesions; Leukocytes (eosinophilic); Life Sciences; Male; Medical Microbiology; Membrane proteins; Metalloproteinase; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microscopy, Electron, Transmission; Mycology; Nucleotides; Perineum; Polymerase chain reaction; Porcupines; Poxviridae Infections; Rural areas; Skin; Skin diseases; Skin lesions; Transmission electron microscopy; Veterinary Microbiology - Short Communication; Virions
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-022-00804-3

Brazilian porcupine poxvirus (BPoPV) is a new poxvirus recently described in porcupines ( Coendou prehensilis ) from Brazil. Herein, we described a free-ranging adult male Coendou ( Sphiggurus ) spinosus rescued after being found lethargic on the ground in a rural area. The animal presented crusty, edematous, and suppurative skin lesions on the face, tail, and perineum, and yellowish ocular secretion. The diagnosis was performed by histopathology, transmission electron microscopy (TEM), PCR, and sequencing. Microscopically, proliferative and necrotizing dermatitis, subacute, multifocal with ballooning degeneration, and eosinophilic intracytoplasmic viral inclusion bodies were observed. TEM confirmed large brick-shaped virions inside the keratinocyte cytoplasm, measuring about 200–280 × 120–180 nm. Partial fragment of intracellular mature virion membrane protein gene and putative metalloproteinase gene was successfully amplified and sequenced, and the strain herein denoted IAL/21 V-102 was classified as BPoPV, showing 99.4% of nucleotide identity to the reference strain UFU/USP001. Enrofloxacin 10% (10 mg/kg) was administered every 24 h through intramuscular injection for 10 days, dipyrone/metamizole (25 mg/kg) every 24 h orally (PO) for 3 days, 0.5 ml (mL) of thymomodulin every 24 h PO for 30 days, and each 48 h for another 15 days. The lesions were cleaned and debrided every 15 days. Seventy-five days after the beginning of the treatment, the cutaneous lesions regressed, the animal gained weight, and was clinically stable. After treatment, the skin biopsy showed only mild epidermal acanthosis, intra-cellular edema, and mild lymphoplasmacytic perivascular dermatitis. No viral particles were observed by TEM and no poxviral DNA was amplified by PCR. This study documents the first case of confirmed and treated BPoPV infection in a hairy dwarf porcupine. The implemented therapeutic plan eliminated the infection and improved the general state of the animal.