The Effect of Irisin on Proliferation, Apoptosis, and Expression of Metastasis Markers in Prostate Cancer Cell Lines

Introduction Irisin is a newly discovered myokine released from skeletal muscle during exercise. The matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that play a key role in the metastatic process via degrading extracellular matrix. The aim of this study was to investigate the ef...

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Bibliographic Details
Published in:Oncology and therapy 2022-12, Vol.10 (2), p.377-388
Main Authors: Saeedi Sadr, Atiye, Ehteram, Hassan, Seyed Hosseini, Elahe, Alizadeh Zarei, Marziyeh, Hassani Bafrani, Hassan, Haddad Kashani, Hamed
Format: Article
Language:English
Subjects:
Internal Medicine
Medicine
Medicine & Public Health
Original Research
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Summary:Introduction Irisin is a newly discovered myokine released from skeletal muscle during exercise. The matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that play a key role in the metastatic process via degrading extracellular matrix. The aim of this study was to investigate the effect of irisin on expression of metastatic markers MMP2 and MMP9 and induced apoptosis in human prostate cancer cells. Methods In this study, we examined the effect of different concentrations of irisin on induced apoptosis and cell viability of two cell lines, LNCaP and DU-145, by using flow cytometry and MTT assay, respectively. The expression of MMP2 and MMP9 genes was also analyzed by real-time PCR after irisin treatment. Data were analyzed using the comparative cycle threshold 2 −∆∆Ct method. Results Cell viability was reduced in both LNCaP and DU-145 cell lines at different concentrations of irisin. However, this decreased cell viability was strongly significant ( p  
ISSN:2366-1070
2366-1089
DOI:10.1007/s40487-022-00194-4