Peptide-based direct electrochemical detection of receptor binding domains of SARS-CoV-2 spike protein in pristine samples

RNA isolation and amplification-free user-friendly detection of SARS-CoV-2 is the need of hour especially at resource limited settings. Herein, we devised the peptides of human angiotensin converting enzyme-2 (hACE-2) as bioreceptor at electrode interface for selective targeting of receptor binding...

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Published in:Sensors and actuators. B, Chemical Chemical, 2023-02, Vol.377, p.133052-133052, Article 133052
Main Authors: Kumar, T.H. Vignesh, Srinivasan, Sowmiya, Krishnan, Vinoth, Vaidyanathan, Rama, Babu, Kannadasan Anand, Natarajan, Sudhakar, Veerapandian, Murugan
Format: Article
Language:English
Subjects:
COVID sensor
Electrochemical biosensor
N-terminal hACE-2 peptide
Peptide biosensor
RNA Isolation-free
SARS-CoV-2 RBD
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Summary:RNA isolation and amplification-free user-friendly detection of SARS-CoV-2 is the need of hour especially at resource limited settings. Herein, we devised the peptides of human angiotensin converting enzyme-2 (hACE-2) as bioreceptor at electrode interface for selective targeting of receptor binding domains (RBD) of SARS-CoV-2 spike protein (SP). Disposable carbon-screen printed electrode modified with methylene blue (MB) electroadsorbed graphene oxide (GO) has been constructed as cost-efficient and scalable platform for hACE-2 peptide-based SARS-CoV-2 detection. In silico molecular docking of customized 25 mer peptides with RBD of SARS-CoV-2 SP were validated by AutoDock CrankPep. N-terminal region of ACE-2 showed higher binding affinity of − 20.6 kcal/mol with 15 H-bond, 9 of which were
ISSN:0925-4005
1873-3077
0925-4005
DOI:10.1016/j.snb.2022.133052