Cytotoxic Evaluation of the New Composite Resin through an Artificial Pulp Chamber

The aim of this study was to analyse the cytocompatibility of Surefil One (SuO) with respect to the release of monomers from the material. The following reference materials were chosen: SDR Flow Plus (SDR, Dentsply Sirona, Konstanz, Germany), One Q Bond (Q, Dentalica, Milan, Italy), and Ketac (K, 3M...

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Bibliographic Details
Published in:BioMed research international 2022-11, Vol.2022, p.1-10
Main Authors: Marigo, Luca, Triestino, Alessio, Castagnola, Raffaella, Vincenzoni, Federica, Cordaro, Massimo, Di Stasio, Enrico, Mordente, Alvaro, Nocca, Giuseppina
Format: Article
Language:English
Subjects:
Acids
Acrylic acid
Adhesives
Biochemical toxicology
Biocompatibility
Bond strength
Cell culture
Chambers
Chemical properties
Composite materials
Composition
Culture media
Cytotoxicity
Dental acrylic resins
Dental research
Dental resins
Dentin
Diameters
Enamel
Fibroblasts
Health aspects
Liquid chromatography
Materials
Molars
Monomers
Permeability
Polymeric composites
Resins
Statistical analysis
Thickness
Variance analysis
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Summary:The aim of this study was to analyse the cytocompatibility of Surefil One (SuO) with respect to the release of monomers from the material. The following reference materials were chosen: SDR Flow Plus (SDR, Dentsply Sirona, Konstanz, Germany), One Q Bond (Q, Dentalica, Milan, Italy), and Ketac (K, 3M-ESPE, USA). Fifteen dentin discs (2 mm thickness and diameter) were obtained from 15 third molars and were used in this study. After dentin disc permeability measurement, murine fibroblasts were grown, and the pulp surface of the dentinal disc was placed in direct contact with the cells immersed in DMEM. The experimental materials were positioned on the occlusal side of each dentinal disc until a uniform thickness of 2 mm was obtained. Then, the discs were inserted into an artificial pulp chamber for 24 hours to assess the cytocompatibility. Afterwards, the moles of monomers leached from the specimens in DMEM were determined using HPLC. Statistical analysis was performed using ANOVA (p
ISSN:2314-6133
2314-6141
DOI:10.1155/2022/5100816