Cloning and Functional Analysis of NoMYB60 Gene Involved in Flavonoid Biosynthesis in Watercress ( Nasturtium officinale R. Br.)

The gene belongs to the R2R3-MYB subfamily, which includes the genes. Although these genes have been shown to respond to heat and drought stresses, their role in flavonoid synthesis remains unclear. In this study, was cloned from watercress and its structure and function were analyzed. Sequence stru...

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Bibliographic Details
Published in:Genes 2022-11, Vol.13 (11), p.2109
Main Authors: Ma, Xiaoqing, Ran, Jiajun, Mei, Guihu, Hou, Xilin, You, Xiong
Format: Article
Language:English
Subjects:
Abscisic acid
Amino acids
Biosynthesis
Cloning
Cloning, Molecular
Conserved sequence
Drought
Flavonoids
Flavonoids - genetics
Gene Expression Regulation, Plant
Gene silencing
Genes
Localization
N-Terminus
Nasturtium - genetics
Nasturtium - metabolism
Nucleotide sequence
Plant Proteins - metabolism
Plasmids
Proteins
Structure-function relationships
Thermal cycling
Transcription factors
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Summary:The gene belongs to the R2R3-MYB subfamily, which includes the genes. Although these genes have been shown to respond to heat and drought stresses, their role in flavonoid synthesis remains unclear. In this study, was cloned from watercress and its structure and function were analyzed. Sequence structure analysis showed that had a highly conserved R2R3 DNA-binding region at the N-terminus. Under the treatment of ABA, SA or MeJA, the expression level of first significantly increased and then decreased, indicating that ABA, SA and MeJA positively regulated . The subcellular localization of NoMYB60-GFP indicated that NoMYB60 was localized in the nuclear region, which is consistent with the molecular characterization of the transcription factor. Gene silencing experiments were also performed to further test the function of . The result showed that virus-induced silencing of affected the expression of enzyme genes in flavonoid synthesis pathways and promoted the synthesis of flavonoids. Moreover, we discovered that NoMYB60 interacts with NoBEH1/2. In this study, provides a reference for research on the regulation mechanism of flavonoid synthesis in Cruciferae and other crops.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes13112109