Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

The in vitro culture technique can be used for micropropagation of medicinal plants as well as for creating genotypes with an improved profile of phytochemical compounds. For this purpose, somaclonal variability may be used for the induction of genetic diversity among regenerants. The paper presents...

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Bibliographic Details
Published in:Genes 2022-11, Vol.13 (11), p.2114
Main Authors: Gawroński, Jacek, Dyduch-Siemińska, Magdalena
Format: Article
Language:English
Subjects:
Benzyladenine
Biomarkers
Codon, Initiator
Drinking water
Experiments
Flavonoids
Genetic analysis
Genetic diversity
Genetic variability
Genetic Variation - genetics
Genotypes
Herbal medicine
Kinetin
Life sciences
Medicinal plants
Micropropagation
Organogenesis
Plant growth
Plantlets
Plants, Medicinal - genetics
Scutellaria baicalensis
Scutellaria baicalensis - genetics
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Summary:The in vitro culture technique can be used for micropropagation of medicinal plants as well as for creating genotypes with an improved profile of phytochemical compounds. For this purpose, somaclonal variability may be used for the induction of genetic diversity among regenerants. The paper presents a protocol for obtaining regenerants by indirect organogenesis and the assessment of their genetic variability with the use of start codon-targeted markers. The most intense process of indirect shoot organogenesis was observed on Murashige and Skoog medium supplemented with kinetin and 6-Benzylaminopurine (0.5 mg × dm each)-7.4 shoot per explant on average. The callogenesis process occurred on the medium supplemented with TDZ, while the medium supplemented with GA allowed for direct shoot organogenesis and was used for the micropropagation of regenerants. In the analysis of plantlets obtained by indirect organogenesis, 11 ScoT markers generated a total of 130 amplicons, 45 of which were polymorphic. This analysis showed genetic diversity of regenerants in relation to the donor plant as well as within them, with mean similarity among the analyzed genotypes at the level of 0.90. This study confirms that the use of in vitro cultures allows for the possibility to generate genetic variability in , which can be effectively revealed with the use of the SCoT marker.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes13112114