Syntheses and immunologic properties of Escherichia coli O157 O-specific polysaccharide and Shiga toxin 1 B subunit conjugates in mice

Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157....

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Bibliographic Details
Published in:Infection and immunity 1999-11, Vol.67 (11), p.6191-6193
Main Authors: KONADU, E, DONOHUE-ROLFE, A, CALDERWOOD, S. B, POZSGAY, V, SHILOACH, J, ROBBINS, J. B, SZU, S. C
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - blood
Bacterial Toxins - immunology
Bacterial Vaccines - immunology
Bacteriology
Biological and medical sciences
Escherichia coli
Escherichia coli O157 - immunology
Female
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
lipopolysaccharides
Mice
Microbial Immunity and Vaccines
Microbiology
O Antigens - immunology
polysaccharides
Shiga toxin
Shiga toxin 1
Shiga Toxins
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Conjugate - immunology
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Summary:Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157. Here, the detoxified LPS was conjugated with improved schemes to the nontoxic B subunit of Stx1. Mice injected with these bivalent conjugates elicited both bactericidal antibodies to E. coli O157 and neutralization antibodies to Stx1.
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.67.11.6191-6193.1999