Integration of Lipidomics and Transcriptomics Reveals the Efficacy and Mechanism of Qige Decoction on NAFLD

The prevalence of nonalcoholic fatty liver disease (NAFLD) is increasing as obesity and diabetes become more common. There are no drugs approved for NAFLD yet. Qige decoction (QGD), a traditional Chinese medicine (TCM) formula, is used for NAFLD and hyperlipidemia treatment in TCM and has shown hypo...

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Published in:Evidence-based complementary and alternative medicine 2022-11, Vol.2022, p.1-13
Main Authors: Fan, Simin, Zhou, Zunming, Ye, Jintong, Li, Yanfang, Huang, Keer, Ke, Xuehong
Format: Article
Language:English
Subjects:
Biosynthesis
Chinese medicine
Cholesterol
Diabetes mellitus
Disease
Drug dosages
Fatty acids
Fatty liver
Gene expression
Glucose
High fat diet
Hyperlipidemia
Insulin
Insulin resistance
Laboratory animals
Lipid metabolism
Lipids
Liver
Liver diseases
Low density lipoprotein
Metabolism
Metabolites
Molecular modelling
Pharmaceutical industry
Steatosis
Traditional Chinese medicine
Transcriptomes
Transcriptomics
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description The prevalence of nonalcoholic fatty liver disease (NAFLD) is increasing as obesity and diabetes become more common. There are no drugs approved for NAFLD yet. Qige decoction (QGD), a traditional Chinese medicine (TCM) formula, is used for NAFLD and hyperlipidemia treatment in TCM and has shown hypolipidemic and hepatoprotective effects. This study tried to interpret the pharmacology and molecular mechanisms of QGD in NAFLD rats. Firstly, the therapeutic effects of QGD on high-fat diet (HFD)-induced NAFLD rats were evaluated. Then, integration of lipidomics and transcriptomics was conducted to explore the possible pathways and targets of QGD against NAFLD. QGD at low dosage (QGL) administration reduced serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) (P
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There are no drugs approved for NAFLD yet. Qige decoction (QGD), a traditional Chinese medicine (TCM) formula, is used for NAFLD and hyperlipidemia treatment in TCM and has shown hypolipidemic and hepatoprotective effects. This study tried to interpret the pharmacology and molecular mechanisms of QGD in NAFLD rats. Firstly, the therapeutic effects of QGD on high-fat diet (HFD)-induced NAFLD rats were evaluated. Then, integration of lipidomics and transcriptomics was conducted to explore the possible pathways and targets of QGD against NAFLD. QGD at low dosage (QGL) administration reduced serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) (P&lt;0.05). Liver histopathology indicated that QGL could alleviate hepatic steatosis. The main differential lipids (DELs) affected by QGD were glycerolipids. KEGG enrichment analysis suggested that the main pathways by which QGD improved NAFLD may be cholesterol metabolism, glycerolipid metabolism, and insulin resistance. Transcriptome sequencing identified 179 upregulated and 194 downregulated mRNAs after QGD treatment. An interaction network based on DELs and differential genes (DEGs) suggested that QGD inhibited hepatic steatosis mainly by reducing hepatic insulin resistance and triglyceride biosynthesis via the PPP1R3C/SIK1/CRTC2 and PPP1R3C/SIK1/SREBP1 signal axis, respectively. These findings indicated that QGD could protect against NAFLD induced by HFD. The improvement of hepatic insulin resistance and the reduction of triglyceride biosynthesis might be the potential mechanisms.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2022/9739032</identifier><language>eng</language><publisher>New York: Hindawi</publisher><subject>Biosynthesis ; Chinese medicine ; Cholesterol ; Diabetes mellitus ; Disease ; Drug dosages ; Fatty acids ; Fatty liver ; Gene expression ; Glucose ; High fat diet ; Hyperlipidemia ; Insulin ; Insulin resistance ; Laboratory animals ; Lipid metabolism ; Lipids ; Liver ; Liver diseases ; Low density lipoprotein ; Metabolism ; Metabolites ; Molecular modelling ; Pharmaceutical industry ; Steatosis ; Traditional Chinese medicine ; Transcriptomes ; Transcriptomics</subject><ispartof>Evidence-based complementary and alternative medicine, 2022-11, Vol.2022, p.1-13</ispartof><rights>Copyright © 2022 Simin Fan et al.</rights><rights>Copyright © 2022 Simin Fan et al. 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subjects Biosynthesis
Chinese medicine
Cholesterol
Diabetes mellitus
Disease
Drug dosages
Fatty acids
Fatty liver
Gene expression
Glucose
High fat diet
Hyperlipidemia
Insulin
Insulin resistance
Laboratory animals
Lipid metabolism
Lipids
Liver
Liver diseases
Low density lipoprotein
Metabolism
Metabolites
Molecular modelling
Pharmaceutical industry
Steatosis
Traditional Chinese medicine
Transcriptomes
Transcriptomics
title Integration of Lipidomics and Transcriptomics Reveals the Efficacy and Mechanism of Qige Decoction on NAFLD
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