Antioxidant and protective effects of a peptide (VTAL) derived from simulated gastrointestinal digestion of protein hydrolysates of Magallana gigas against acetaminophen-induced HepG2 cells

Oxidative stress is an automatic mechanism responsible for the commencement and continuance of liver injury. In this study, an antioxidative peptide Val-Thr-Ala-Leu (VTAL) was purified from simulated gastrointestinal digestion of protein hydrolysates of the triploid oyster Magallana gigas . Signific...

Full description

Saved in:
Bibliographic Details
Published in:Fisheries science 2023-01, Vol.89 (1), p.71-81
Main Authors: Ulagesan, Selvakumari, Park, Su-Jin, Nam, Taek-Jeong, Choi, Youn-Hee
Format: Article
Language:English
Subjects:
Acetaminophen
Amino acids
Analgesics
Antioxidants
Biomedical and Life Sciences
Cell proliferation
Cell viability
Cells
Digestion
Digestive system
Fish & Wildlife Biology & Management
Food Science
Freshwater & Marine Ecology
Gastrointestinal tract
Hepatocytes
Hydrolysates
Hydrophobicity
Life Sciences
Liver cancer
Magallana gigas
Marine molluscs
Original
Original Article
Oxidative stress
Peptides
Proliferation
Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oxidative stress is an automatic mechanism responsible for the commencement and continuance of liver injury. In this study, an antioxidative peptide Val-Thr-Ala-Leu (VTAL) was purified from simulated gastrointestinal digestion of protein hydrolysates of the triploid oyster Magallana gigas . Significant antioxidant activity was identified, as well as a protective effect against acetaminophen (APAP)-induced human liver cancer (HepG2) cells. The results suggested that the antioxidant activity improved in a dose-dependent manner. The highest cell viability (88.105 ± 3.62%) was observed in 15 mM APAP-induced cells when treated with 25 μg/mL M. gigas peptide [M.g (pep)]. The peptide sequences include hydrophobic amino acids, which could be responsible for its chemoprotective and antioxidant activities. Treatment with M.g (pep) significantly promoted the proliferation of HepG2 cells, thus protecting them against APAP and imbuing them with significant antioxidant capacity. M.g (pep) could be beneficial for treating drug-induced oxidative stress and liver damage. Additionally, M.g (pep) could serve as an alternative to synthetic antioxidant drugs.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-022-01639-5