Down-regulation of miR-140-3p is a cause of the interlukin-13-induced up-regulation of RhoA protein in bronchial smooth muscle cells

The current study aimed to determine the role of a microRNA (miRNA), miR-140-3p, in the control of RhoA expression in bronchial smooth muscle cells (BSMCs). In cultured human BSMCs, incubation with interleukin-13 (IL-13) caused an up-regulation of RhoA protein concurrently with a down-regulation of...

Full description

Saved in:
Bibliographic Details
Published in:Small GTPases 2022, Vol.13 (1), p.1-6
Main Authors: Chiba, Yoshihiko, Ando, Yusuke, Kato, Yasuna, Hanazaki, Motohiko, Sakai, Hiroyasu
Format: Article
Language:English
Subjects:
Asthma - genetics
Asthma - metabolism
Brief Report
Down-Regulation
Humans
Interleukin-13 - genetics
Interleukin-13 - metabolism
Interleukin-13 - pharmacology
MicroRNAs - genetics
MicroRNAs - metabolism
Myocytes, Smooth Muscle - metabolism
rhoA GTP-Binding Protein - genetics
rhoA GTP-Binding Protein - metabolism
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The current study aimed to determine the role of a microRNA (miRNA), miR-140-3p, in the control of RhoA expression in bronchial smooth muscle cells (BSMCs). In cultured human BSMCs, incubation with interleukin-13 (IL-13) caused an up-regulation of RhoA protein concurrently with a down-regulation of miR-140-3p. Transfection of the cells with a miR-140-3p inhibitor caused an increase in basal RhoA protein level. Although a mimic of miR-140-3p had little effect on the basal RhoA level, its treatment inhibited the IL-13-induced up-regulation of RhoA. These findings suggest that RhoA expression is negatively regulated by miR-140-3p, and that the negative regulation is inhibited by IL-13 to cause an up-regulation of RhoA protein in BSMCs.
ISSN:2154-1248
2154-1256
DOI:10.1080/21541248.2021.1872318