Micro-Raman spectroscopy study of blood samples from myocardial infarction patients

Acute myocardial infarction (MI) is found to be a major causative factor for global mortality and morbidity. This situation demands necessity of developing efficient and rapid diagnostic tools to detect acute MI. Raman spectroscopy is a non-destructive optical diagnostic technique, which has high po...

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Bibliographic Details
Published in:Lasers in medical science 2022-12, Vol.37 (9), p.3451-3460
Main Authors: John, Reena V., Devasia, Tom, N., Mithun, Lukose, Jijo, Chidangil, Santhosh
Format: Article
Language:English
Subjects:
Aspirin
Blood
Dentistry
Heart attacks
Inflammation
Lasers
Medicine
Medicine & Public Health
Morbidity
Myocardial infarction
Nondestructive testing
Optical Devices
Optics
Original
Original Article
Patients
Phenylalanine
Photonics
Quantum Optics
Raman spectra
Raman spectroscopy
Sample preparation
Spectroscopy
Spectrum analysis
Tetrahydrobiopterin
Tyrosine
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Summary:Acute myocardial infarction (MI) is found to be a major causative factor for global mortality and morbidity. This situation demands necessity of developing efficient and rapid diagnostic tools to detect acute MI. Raman spectroscopy is a non-destructive optical diagnostic technique, which has high potential in probing biochemical changes in clinical samples during initiation and progress of diseases. In this work, blood was taken as the sample to examine inflammation in acute MI patients using Raman spectroscopy. Ratio of Raman peak intensities that corresponds to phenylalanine (1000 cm −1 ) and tyrosine (825 cm −1 ) can facilitate indirect information about tetrahydrobiopterin (BH4) availability, which can indicate inflammatory status in patients. This ratio obtained was higher for MI patients in comparison with control subjects. The decrease in phenylalanine and tyrosine ratio (Phe-Tyr ratio) is attributed to the prognosis of standard of care (medications like antiplatelets including aspirin, statin and revascularisation) leading to inflammation reduction. Phe-Tyr ratio estimated from the Raman spectra of blood can be exploited as a reliable method to probe inflammation due to MI. The method is highly objective, require only microliters of sample and minimal sample preparation, signifying its clinical utility.
ISSN:1435-604X
0268-8921
1435-604X
DOI:10.1007/s10103-022-03604-1