Enhancement of recombinant human IL-24 (rhIL-24) protein production from site-specific integrated engineered CHO cells by sodium butyrate treatment

• Published in: Bioprocess and biosystems engineering 2022-12, Vol.45 (12), p.1979-1991
• Main Authors: Hua, Jilei, Xu, Hanli, Zhang, Yao, Ge, Jianlin, Liu, Mengzhe, Wang, Yuqi, Wei, Yuexian, Shi, Yinan, Hou, LingLing, Jiang, Hong
• Format: Article
• Language: English
• Subjects: Ammonia; Anticancer properties; Antitumor activity; Apoptosis; Biotechnology; Cell culture; Cell cycle; Cell proliferation; Chemistry; Chemistry and Materials Science; Culture; Environmental Engineering/Biotechnology; Food Science; G1 phase; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Interleukin 24; Interleukins; Lactic acid; Mammalian cells; Proteins; Research Paper; Sodium; Sodium butyrate; Suspension culture; Toxicity; Tumor cells
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-022-02801-0

Interleukin-24 (IL-24) has specific inhibitory effects on the proliferation of various tumor cells with almost no toxicity to normal cells. The antitumor activity of recombinant human IL-24 protein produced in mammalian cells is much higher than that of bacteria, but its expression level is extremely low. Sodium butyrate (NaBu) was utilized as a media additive to increase protein expression in Chinese hamster ovary cells. The site-specific integrated engineered cells FCHO/IL-24 were treated with NaBu under different culture conditions (10% and 0.5% serum adherent culture, 0.5% serum suspension culture). First, 3 days of 1 mmol/L NaBu treatment significantly increased rhIL-24 expression level in FCHO/IL-24 cells by 119.94 ± 1.5% (** p