Identification of the operon for the sorbitol (glucitol) phosphoenolpyruvate:sugar phosphotransferase system in Streptococcus mutans

Transposon mutagenesis and marker rescue were used to isolate and identify an 8.5-kb contiguous region containing six open reading frames constituting the operon for the sorbitol P-enolpyruvate phosphotransferase transport system (PTS) of Streptococcus mutans LT11. The first gene, srlD, codes for so...

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Bibliographic Details
Published in:Infection and immunity 2000-02, Vol.68 (2), p.925-930
Main Authors: BOYD, D. A, THEVENOT, T, GUMBMANN, M, HONEYMAN, A. L, HAMILTON, I. R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacillus
Bacteriology
Biological and medical sciences
Carbohydrate Dehydrogenases - genetics
DNA Transposable Elements
DNA, Bacterial - chemistry
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genes, Regulator
Genetics
Microbiology
Molecular and Cellular Pathogenesis
Molecular Sequence Data
Open Reading Frames
Operon
Phosphoenolpyruvate Sugar Phosphotransferase System - genetics
Sorbitol - metabolism
Sorbitol P-enolpyruvate phosphotransferase
Sorbitol-6-phosphate 2-dehydrogenase
srlA gene
srlB gene
srlD gene
srlE gene
srlM gene
srlR gene
SrlR protein
Streptococcus mutans
Streptococcus mutans - genetics
Streptococcus mutans - growth & development
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Summary:Transposon mutagenesis and marker rescue were used to isolate and identify an 8.5-kb contiguous region containing six open reading frames constituting the operon for the sorbitol P-enolpyruvate phosphotransferase transport system (PTS) of Streptococcus mutans LT11. The first gene, srlD, codes for sorbitol-6-phosphate dehydrogenase, followed downstream by srlR, coding for a transcriptional regulator; srlM, coding for a putative activator; and the srlA, srlE, and srlB genes, coding for the EIIC, EIIBC, and EIIA components of the sorbitol PTS, respectively. Among all sorbitol PTS operons characterized to date, the srlD gene is found after the genes coding for the EII components; thus, the location of the gene in S. mutans is unique. The SrlR protein is similar to several transcriptional regulators found in Bacillus spp. that contain PTS regulator domains (J. Stülke, M. Arnaud, G. Rapoport, and I. Martin-Verstraete, Mol. Microbiol. 28:865-874, 1998), and its gene overlaps the srlM gene by 1 bp. The arrangement of these two regulatory genes is unique, having not been reported for other bacteria.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.68.2.925-930.2000