Fermented Taiwanofungus camphoratus Extract Ameliorates Psoriasis-Associated Response in HaCaT Cells via Modulating NF-κB and mTOR Pathways

• Published in: International journal of molecular sciences 2022-11, Vol.23 (23), p.14623
• Main Authors: Shen, Jia-Wei, Wu, Po-Yuan, Kuo, Yueh-Hsiung, Chang, Qiao-Xin, Wen, Kuo-Ching, Chiang, Hsiu-Mei
• Format: Article
• Language: English
• Subjects: Anti-Inflammatory Agents - pharmacology; Antioxidants; Assaying; Autoimmune diseases; Biological Products - pharmacology; Cell growth; Cell proliferation; Cytokines; Drug development; HaCaT Cells - drug effects; HaCaT Cells - metabolism; Humans; Hypotheses; Inflammation; Interleukin 17; Interleukin-17 - metabolism; Intracellular; Keratinocytes; Keratinocytes - drug effects; Keratinocytes - metabolism; Kinases; NF-kappa B - metabolism; Oxidative stress; Phosphorylation; Proteins; Psoriasis; Psoriasis - pathology; Scavenging; Skin; Skin diseases; Taiwanofungus camphoratus; TOR protein; TOR Serine-Threonine Kinases - metabolism; Tumor necrosis factor-TNF
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232314623

Psoriasis is a chronic autoimmune disease, and until now, it remains an incurable disease. Therefore, the development of new drugs or agents that ameliorate the disease will have marketing potential. (TC) is a specific fungus in Taiwan. It is demonstrated to have anticancer, anti-inflammation, and hepatoprotective effects. However, the effects of TC fermented extract on psoriasis are under investigation. In this research, we studied the ability of TC on antioxidative activity and the efficacy of TC on interleukin-17 (IL-17A)-induced intracellular oxidative stress, inflammation-relative, and proliferation-relative protein expression in human keratinocytes. The results of a DPPH radical scavenging assay, reducing power assay, and hydroxyl peroxide inhibition assay indicated that TC has a potent antioxidant ability. Furthermore, TC could reduce IL-17A-induced intracellular ROS generation and restore the NADPH level. In the investigation of pathogenesis, we discovered TC could regulate inflammatory and cell proliferation pathways via -IKKα/ -p65 and -mTOR/ -p70S6k signaling pathways in human keratinocytes. In conclusion, TC showed characteristics such as antioxidant, anti-inflammatory, and anti-psoriatic-associated responses. It is expected to be developed as a candidate for oxidative-stress-induced skin disorders or psoriasis treatment.