Improved Stability and In Vitro Anti-Arthritis Bioactivity of Curcumin-Casein Nanoparticles by Ultrasound-Driven Encapsulation

Curcumin possesses beneficial biological functions, namely anti-inflammation and anti-diabetic functions. However, due to its low solubility and crystallinity, its applications are limited. In this work, curcumin was encapsulated in casein micelles in order to form curcumin-casein nanoparticles by u...

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Bibliographic Details
Published in:Nutrients 2022-12, Vol.14 (23), p.5192
Main Authors: Li, Kexin, Zhang, Yahui, Hao, Xueyan, Xie, Dongchao, Wang, Chongchong, Zhang, Haihua, Jin, Peng, Du, Qizhen
Format: Article
Language:English
Subjects:
Arthritis
Bioavailability
Biological activity
Body temperature
Casein
Caseins - chemistry
Caseins - pharmacology
Cell proliferation
Cold storage
Crystallinity
Curcumin
Curcumin - chemistry
Curcumin - pharmacology
Cytokines
Diabetes mellitus
Encapsulation
Endocytosis
Fibroblasts
Food
Humans
Hydrophobicity
Industrial development
Inflammation
Micelles
Nanoparticles
Nanoparticles - chemistry
Osteoarthritis
Particle Size
Proteins
Room temperature
Sodium
Solubility
Stability
Tumor necrosis factor-TNF
Ultrasonic imaging
Ultrasonic processing
Ultrasound
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Summary:Curcumin possesses beneficial biological functions, namely anti-inflammation and anti-diabetic functions. However, due to its low solubility and crystallinity, its applications are limited. In this work, curcumin was encapsulated in casein micelles in order to form curcumin-casein nanoparticles by ultrasound treatment (5 min). The ultrasound treatment induced the entry of the hydrophobic groups to the inner micelles and the polar sulfydryl groups to the surface of the micelles in order to form compact curcumin-casein nanoparticles of an appropriate size (100-120 nm) for cellular endocytosis. The product exhibited excellent stability during 8 months of cold storage, 6 days at room temperature, and 2 days at body temperature. Advanced in vitro experiments demonstrated that curcumin-casein nanoparticles displayed significantly greater inhibitory activity against the proliferation and proinflammatory cytokines of human fibroblast-like synoviocyte-osteo arthritis (HFLS-OA) cells and HFLS-rheumatoid (RA) cells than native curcumin due to better cellular uptake as a result of the low crystallinity and the appropriate nano-size of the nano-form. The results provide a reference for the use of ultrasound treatment to encapsulate other drug molecules and curcumin-casein nanoparticles as potential treatment for arthritis.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu14235192