Intracellular trafficking and killing of Streptococcus pneumoniae by human alveolar macrophages are influenced by opsonins

• Published in: Infection and immunity 2000-04, Vol.68 (4), p.2286-2293
• Main Authors: GORDON, S. B, IRVING, G. R. B, LAWSON, R. A, LEE, M. E, READ, R. C
• Format: Article
• Language: English
• Subjects: Antigens, CD - immunology; Antigens, CD - metabolism; Bacterial Adhesion; Bacteriology; Biological and medical sciences; Bronchoalveolar Lavage Fluid - immunology; Cells, Cultured; Colony Count, Microbial; Fundamental and applied biological sciences. Psychology; Humans; Immunoglobulin G - immunology; Kinetics; LAMP-1 protein; Lysosomal Membrane Proteins; lysosome-associated membrane protein; Macrophages, Alveolar - immunology; Membrane Glycoproteins - immunology; Membrane Glycoproteins - metabolism; Microbiology; Microscopy, Fluorescence; Molecular and Cellular Pathogenesis; Opsonin Proteins - immunology; opsonins; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Phagocytosis; Pneumonia, Pneumococcal - immunology; Receptors, Complement 3b - immunology; Streptococcus pneumoniae; Streptococcus pneumoniae - immunology; Time Factors
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.68.4.2286-2293.2000

Human alveolar macrophages (HAM) are the major resident phagocytic cells of the gas-exchanging areas of the lung. Following contact with macrophages, bacteria enter phagosomes, which gradually acquire the characteristics of terminal phagolysosomes, with incorporation of lysosome-associated membrane protein (LAMP). We measured the binding of type 1 Streptococcus pneumoniae to the surface of HAM and then measured subsequent internalization and phagosomal incorporation of LAMP-1 under various opsonic conditions. Opsonization with serum containing immunoglobulin resulted in significantly greater binding of pneumococci to HAM compared with opsonization with immunoglobulin G (IgG)-depleted serum containing complement, which in turn resulted in marginally increased binding over that observed in the absence of opsonization. Internalization of opsonized S. pneumoniae gradually increased to a maximum of 20% of bound bacteria by 120 min of warm incubation, with 20% of internalized pneumococci being localized within LAMP-containing compartments by 80 min. Internalization of opsonized S. pneumoniae by HAM correlated with a reduction of bacterial viability. When inocula were adjusted so that pneumococcal binding under different conditions was equalized, subsequent internalization, trafficking to LAMP-containing compartments, and reduction of bacterial viability were less efficient in the absence of opsonization than that observed following opsonization with adsorbed or IgG-replete adsorbed serum. Once bound to the surface of HAM, pneumococci opsonized with adsorbed serum with or without IgG were internalized, processed, and killed equally well. In conclusion, binding, intracellular trafficking, and killing of S. pneumoniae by HAM are each significantly increased by opsonization with serum containing immunogloblin and/or complement.