The Upper Respiratory Tract Microbiome Network Impacted by SARS-CoV-2

• Published in: Microbial ecology 2023-08, Vol.86 (2), p.1428-1437
• Main Authors: Li, Wendy, Ma, Zhanshan (Sam)
• Format: Article
• Language: English
• Subjects: Animals; Archaea; Bacteria; Bacteria - genetics; Biomedical and Life Sciences; Chiroptera; Community composition; Composition; Coronaviruses; COVID-19; Ecology; Gene sequencing; Geoecology/Natural Processes; Human Microbiome; Humans; Life Sciences; Metagenomics; Microbial Ecology; Microbiology; Microbiomes; Microbiota - genetics; Microorganisms; Nature Conservation; Nucleic acids; Pathogens; Respiratory System; Respiratory tract; RNA; RNA viruses; SARS-CoV-2 - genetics; Severe acute respiratory syndrome coronavirus 2; Species composition; Statistical analysis; Translocation; Viral diseases; Viruses; Water Quality/Water Pollution
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-022-02148-9

The microbiome of upper respiratory tract (URT) acts as a gatekeeper to respiratory health of the host. However, little is still known about the impacts of SARS-CoV-2 infection on the microbial species composition and co-occurrence correlations of the URT microbiome, especially the relationships between SARS-CoV-2 and other microbes. Here, we characterized the URT microbiome based on RNA metagenomic-sequencing datasets from 1737 nasopharyngeal samples collected from COVID-19 patients. The URT-microbiome network consisting of bacteria, archaea, and RNA viruses was built and analyzed from aspects of core/periphery species, cluster composition, and balance between positive and negative interactions. It is discovered that the URT microbiome in the COVID-19 patients is enriched with Enterobacteriaceae , a gut associated family containing many pathogens. These pathogens formed a dense cooperative guild that seemed to suppress beneficial microbes collectively. Besides bacteria and archaea, 72 eukaryotic RNA viruses were identified in the URT microbiome of COVID-19 patients. Only five of these viruses were present in more than 10% of all samples, including SARS-CoV-2 and a bat coronavirus (i.e., BatCoV BM48-31) not detected in humans by routine means. SARS-CoV-2 was inhibited by a cooperative alliance of 89 species, but seems to cooperate with BatCoV BM48-31 given their statistically significant, positive correlations. The presence of cooperative bat-coronavirus partner of SARS-CoV-2 (BatCoV BM48-31), which was previously discovered in bat but not in humans to the best of our knowledge, is puzzling and deserves further investigation given their obvious implications. Possible microbial translocation mechanism from gut to URT also deserves future studies.