Vanin 1 Gene Role in Modulation of iNOS/MCP-1/TGF-β1 Signaling Pathway in Obese Diabetic Patients

• Published in: Journal of inflammation research 2022, Vol.15, p.6745-6759
• Main Authors: Mosaad, Yasser O, Hussein, Mohammed Abdalla, Ateyya, Hayam, Mohamed, Ahmed H, Ali, Ali A, Ramadan Youssuf, Alaa, Wink, Michael, El-Kholy, Amal A
• Format: Article
• Language: English
• Subjects: Abdomen; Body mass index; Body weight; Cardiology; Cardiovascular disease; Computed tomography; Diabetes; Diabetes mellitus; Fatty liver; Gene expression; Glucose; Hemoglobin; High density lipoprotein; Inflammation; Inflammatory bowel disease; Insulin; Insulin resistance; Liver; Metabolic syndrome; Monocyte chemoattractant protein 1; Nitric-oxide synthase; Obesity; Original Research; Overweight; Oxidative stress; Pathogenesis; Plasma; Plasma levels; Signal transduction; Superoxide dismutase; Teaching hospitals; Transforming growth factor-b1; Tumor necrosis factor-α; Ultrasonic imaging; Vanin-1 gene
• ISSN: 1178-7031; 1178-7031
• DOI: 10.2147/JIR.S386506

Cysteamine, a powerful endogenous antioxidant, is produced mostly by the vanin-1 with pantetheinase activity. With regard to glycemic, inflammatory, and redox factors, the current study sought to evaluate the association between the expression of the vanin-1 gene, oxidative stress, and inflammatory and iNOS signaling pathway in obese diabetic patients. We enrolled 67 male subjects with an average age of 53.5 ± 5.0 years, divided into 4 groups according to the WHO guideline. We determined their plasma levels of glucose, insulin, IRI, HbA1c, TC, TG, HDL-C, TNF- α, MCP-1, TGF-β1, SOD, CAT, and TBARs, as well as expression of the iNOS and Vanin1 genes. Overweight and obese class I and II diabetics had significantly higher levels of plasma glucose, insulin, HbA1c, TNF-α, MCP-1, TGF-β1, CAT, and TBAR as well as iNOS and vanin-1 gene expression compared to healthy control individuals. In addition, as compared to healthy control individuals, overweight obese class I and II diabetics' plasma HDL-C levels and blood SOD activity were significantly lower. In addition, ultrasound and computed tomography showed that the presence of a mild obscuring fatty liver with mild hepatic echogenicity appeared in overweight, class I and II obese diabetic patients. These findings provide important information for understanding the correlation between Vanin 1 and glycemic, inflammatory, and redox factors in obese patients. Furthermore, US and CT analysis were performed to visualize the observed images of fatty liver due to obesity.