Combining subsidiary and synchronous approaches for concurrent spectrofluorimetric assurance of lopinavir and ritonavir in tablets utilized in convention for treatment of coronavirus infection (COVID-19) and biological fluids

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2021-12, Vol.262, p.120066-120066, Article 120066
• Main Authors: Salem, Hesham, Samir, Ebtihal, Abdelaziz, Amany, Alaa El-din, Mahmoud, Abdelgaleel, Mahmoud, Zein, Dina, Madian, Hoda
• Format: Article
• Language: English
• Subjects: COVID-19; Fluorescence spectroscopy; Lopinavir; Ritonavir; Spiked human plasma; Synchronous
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2021.120066

[Display omitted] •Determination of lopinavir and ritonavir down to biological concentration level has been carried out. The determination is based on increasing the selectivity of the spectrofluorimetric technique by combining both derivative and synchronous spectrofluorimetric approaches.•Application of of the method was successfully carried out in the commercial tablets and spiked plasma with good agreement with the comparison methods. In this think about, assurance of lopinavir and ritonavir down to organic concentration level has been carried out. The assurance is based on expanding the selectivity of the spectrofluorimetric procedure by combining both subordinate and synchronous spectrofluorimetric approaches, which allow effective estimation of lopinavir at 248.8 nm and ritonavir at 300.1 nm within the nearness of each other at Δλ of 60 nm. Worldwide Conference on Harmonization approval rules were taken after to completely approve the strategy, and linearity was gotten for the two drugs over the extend of 0.4–2.4 µg mL−1 for Lopinavir and 0.1–0.6 µg mL−1 for ritonavir. Application of of the strategy was successfully carried out within the commercial tablets with great understanding with the comparison strategies. As the detection limits were down to 0.133 and 0.022 µg mL−1 and quantitation limits were 0.395 and 0.068 µg mL−1 for lopinavir and ritonavir, individually; the in vivo assurance of lopinavir and ritonavir in spiked plasma tests was pertinent. The rate recuperations in natural tests were 99.10 ± 0.77 and 99.54 ± 0.60 for lopinavir and ritonavir, individually. Water was utilized as the ideal weakening dissolvable within the proposed strategy which includes an eco-friendly justify.