Transcriptomics Analysis Reveals a More Refined Regulation Mechanism of Methylation in a Drought-Tolerant Variety of Potato

Whether DNA methylation modification affects the gene transcription and expression of potatoes under drought stress is still unknown. In this study, we used comparative transcriptomics to explore the expression pattern of related genes of the drought-tolerant variety Qingshu 9 (Q) and the drought-se...

Full description

Saved in:
Bibliographic Details
Published in:Genes 2022-11, Vol.13 (12), p.2260
Main Authors: Bi, Zhenzhen, Wang, Yihao, Li, Pengcheng, Li, Chengju, Liu, Yindu, Sun, Chao, Yao, Panfeng, Liu, Yuhui, Liu, Zhen, Bai, Jiangping
Format: Article
Language:English
Subjects:
Analysis
Carbohydrate metabolism
DNA biosynthesis
DNA methylation
DNA Methylation - genetics
Drought
Drought resistance
Droughts
Gene expression
Genetic aspects
Genetic transcription
Glutathione
Kinases
Mannitol
Methylation
Photosynthesis
Physiological aspects
Plant growth
Potatoes
Signal transduction
Solanum tuberosum - physiology
Transcriptome - genetics
Transcriptomics
Varieties
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Whether DNA methylation modification affects the gene transcription and expression of potatoes under drought stress is still unknown. In this study, we used comparative transcriptomics to explore the expression pattern of related genes of the drought-tolerant variety Qingshu 9 (Q) and the drought-sensitive variety Atlantic (A) under drought stress and DNA methylation inhibitor treatment. The results showed that there was a significant difference in the number of DEGs between the two varieties' responses to mannitol and 5-azad C, especially when they were co-treated with two reagents, and the gene expression of Q was more sensitive to mannitol after two hours. Furthermore, we found that these differentially expressed genes (DEGs) were significantly enriched in DNA replication, transcription, translation, carbohydrate metabolism, photosynthesis, signal transduction, and glutathione metabolism. These results indicate that the difference in the background of methylation leads to the difference in drought resistance of the two varieties. The complexity of the DNA methylation of variety Q might be higher than that of variety A, and the method of methylation regulation is more refined. This study systematically expands the understanding of the molecular mechanism wherein DNA methylation regulates the response to drought stress.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes13122260