Early induction of gamma interferon and interleukin-10 production in draining lymph nodes from mice infected with Borrelia burgdorferi

Lymph node (LN) cells from C3H/HeJ mice (Lyme disease susceptible) infected for 1 week with Borrelia burgdorferi strain JD1 produced higher levels of gamma interferon (IFN-gamma) when stimulated in vitro with B. burgdorferi spirochetes than equivalent cells from B. burgdorferi-infected C57BL/6J mice...

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Bibliographic Details
Published in:Infection and immunity 2000-12, Vol.68 (12), p.7162-7165
Main Authors: GANAPAMO, Frederic, DENNIS, Vida A, PHILIPP, Mario T
Format: Article
Language:English
Subjects:
Animals
Bacterial diseases
Biological and medical sciences
Borrelia burgdorferi
Enzyme-Linked Immunosorbent Assay
Experimental bacterial diseases and models
Experimental protozoal diseases and models
Female
Fundamental and applied biological sciences. Psychology
g-Interferon
Infectious diseases
Interferon-gamma - biosynthesis
Interleukin-10 - biosynthesis
Interleukin-4 - biosynthesis
Lyme Disease - immunology
Lymph Nodes - metabolism
Medical sciences
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Microbial Immunity and Vaccines
Microbiology
Parasitic diseases
Protozoal diseases
Species Specificity
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Summary:Lymph node (LN) cells from C3H/HeJ mice (Lyme disease susceptible) infected for 1 week with Borrelia burgdorferi strain JD1 produced higher levels of gamma interferon (IFN-gamma) when stimulated in vitro with B. burgdorferi spirochetes than equivalent cells from B. burgdorferi-infected C57BL/6J mice (disease resistant). The interleukin-10 (IL-10) levels were comparable in the two strains, whereas the IL-4 levels were below detection limits. B. burgdorferi-stimulated LN cells from C57BL/6J mice produced significantly higher levels of IFN-gamma in the presence of neutralizing anti-IL-10 antibody than cells cultured with B. burgdorferi alone. No effect of IL-10 neutralization on IFN-gamma production by LN cells from C3H/HeJ mice was observed. Neutralizing antibody to IFN-gamma had no effect on the production of IL-10 by LN cells from C57BL/6J mice. A slight decrease in IL-10 production was detected in culture supernatants of equivalent cells from C3H/HeJ mice. The differential effect of IL-10 on IFN-gamma production in C57BL/6J and C3H/HeJ mice suggests that IL-10 is probably involved in the regulation of IFN-gamma production by LN cells during infection and may be at the root of the differential susceptibility to Lyme arthritis in these two strains of mice.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.68.12.7162-7165.2000