A Novel Approach for the Manufacturing of Gelatin-Methacryloyl

Gelatin and its derivatives contain cell adhesion moieties as well as sites that enable proteolytic degradation, thus allowing cellular proliferation and migration. The processing of gelatin to its derivatives and/or gelatin-containing products is challenged by its gelation below 30 ∘C. In this stud...

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Bibliographic Details
Published in:Polymers 2022-12, Vol.14 (24), p.5424
Main Authors: Grijalva Garces, David, Radtke, Carsten Philipp, Hubbuch, Jürgen
Format: Article
Language:English
Subjects:
Biocompatibility
Cell adhesion
Cell culture
Collagen
Fibroblasts
Gelatin
Hydrogels
Manufacturing
Mechanical properties
Molecular weight
Polymers
Proteins
Room temperature
Sodium
Sol-gel processes
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Summary:Gelatin and its derivatives contain cell adhesion moieties as well as sites that enable proteolytic degradation, thus allowing cellular proliferation and migration. The processing of gelatin to its derivatives and/or gelatin-containing products is challenged by its gelation below 30 ∘C. In this study, a novel strategy was developed for the dissolution and subsequent modification of gelatin to its derivative gelatin-methacryloyl (GelMA). This approach was based on the presence of urea in the buffer media, which enabled the processing at room temperature, i.e., lower than the sol-gel transition point of the gelatin solutions. The degree of functionalization was controlled by the ratio of reactant volume to the gelatin concentration. Hydrogels with tailored mechanical properties were produced by variations of the GelMA concentration and its degree of functionalization. Moreover, the biocompatibility of hydrogels was assessed and compared to hydrogels formulated with GelMA produced by the conventional method. NIH 3T3 fibroblasts were seeded onto hydrogels and the viability showed no difference from the control after a three-day incubation period.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym14245424