Time-Dependent Collagen Fibered Structure in the Early Distraction Callus: Imaging Characterization and Mathematical Modeling

Collagen is a ubiquitous protein present in regenerating bone tissues that experiences multiple biological phenomena during distraction osteogenesis until the deposition of phosphate crystals. This work combines fluorescence techniques and mathematical modeling to shed light on the mechano-structura...

Full description

Saved in:
Bibliographic Details
Published in:Annals of biomedical engineering 2022-12, Vol.50 (12), p.1798-1809
Main Authors: Blázquez-Carmona, Pablo, Sanz-Herrera, José A., Mora-Macías, Juan, Morgaz, Juan, Domínguez, Jaime, Reina-Romo, Esther
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Biomedicine
Biophysics
Bone Regeneration
Bony Callus - diagnostic imaging
Callus
Classical Mechanics
Collagen
Crystals
Densification
Density
Diagnostic Imaging
Distraction osteogenesis
Fiber orientation
Fibers
Mathematical analysis
Mathematical models
Maturation
Metatarsus
Mineralization
Models, Theoretical
Original
Original Article
Osteogenesis
Osteogenesis, Distraction - methods
Sheep
Stiffening
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Collagen is a ubiquitous protein present in regenerating bone tissues that experiences multiple biological phenomena during distraction osteogenesis until the deposition of phosphate crystals. This work combines fluorescence techniques and mathematical modeling to shed light on the mechano-structural processes behind the maturation and accommodation-to-mineralization of the callus tissue. Ovine metatarsal bone calluses were analyzed through confocal images at different stages of the early distraction osteogenesis process, quantifying the fiber orientation distribution and mean intensity as fiber density measure. Likewise, a mathematical model based on the experimental data was defined to micromechanically characterize the apparent stiffening of the tissue within the distracted callus. A reorganization of the fibers around the distraction axis and increased fiber density were found as the bone fragments were gradually separated. Given the degree of significance between the mathematical model and previous in vivo data, reorganization, densification, and bundle maturation phenomena seem to explain the apparent mechanical maturation observed in the tissue theoretically.
ISSN:0090-6964
1573-9686
DOI:10.1007/s10439-022-02992-3