Light-Induced Condensation of Biofunctional Molecules around Targeted Living Cells to Accelerate Cytosolic Delivery

The light-induced force and convection can be enhanced by the collective effect of electrons (superradiance and red shift) in high-density metallic nanoparticles, leading to macroscopic assembly of target molecules. We here demonstrate application of the light-induced assembly for drug delivery syst...

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Bibliographic Details
Published in:Nano letters 2022-12, Vol.22 (24), p.9805-9814
Main Authors: Nakase, Ikuhiko, Miyai, Moe, Noguchi, Kosuke, Tamura, Mamoru, Yamamoto, Yasuyuki, Nishimura, Yushi, Omura, Mika, Hayashi, Kota, Futaki, Shiroh, Tokonami, Shiho, Iida, Takuya
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Drug Delivery Systems
Gold - chemistry
Letter
Light
Metal Nanoparticles
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Summary:The light-induced force and convection can be enhanced by the collective effect of electrons (superradiance and red shift) in high-density metallic nanoparticles, leading to macroscopic assembly of target molecules. We here demonstrate application of the light-induced assembly for drug delivery system with enhancement of cell membrane accumulation and penetration of biofunctional molecules including cell-penetrating peptides (CPPs) with superradiance-mediated photothermal convection. For induction of photothermal assembly around targeted living cells in cell culture medium, infrared continuous-wave laser light was focused onto high-density gold-particle-bound glass bottom dishes exhibiting plasmonic superradiance or thin gold-film-coated glass bottom dishes. In this system, the biofunctional molecules can be concentrated around the targeted living cells and internalized into them only by 100 s laser irradiation. Using this simple approach, we successfully achieved enhanced cytosolic release of the CPPs and apoptosis induction using a pro-apoptotic domain with a very low peptide concentration (nM level) by light-induced condensation.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.2c02437