Minding the matrix: The importance of inoculum suspensions on finger transfer efficiency of virus

Aims The aim of this study was to determine how the transfer efficiency of MS‐2 coliphage from the toilet seat to hands and fingertip to lip differs according to the suspension of the inoculum. Methods and Results Hands were sampled after lifting a toilet seat which was inoculated with MS‐2 on the u...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied microbiology 2022-11, Vol.133 (5), p.3083-3093
Main Authors: Abney, Sarah E., Wilson, Amanda M., Ijaz, M. Khalid, McKinney, Julie, Reynolds, Kelly A., Gerba, Charles P.
Format: Article
Language:English
Subjects:
Finger
finger transfer
Fingers
fomites
Humans
Infections
Inoculum
matrices
Microorganisms
Norovirus
Original
Phosphates
Risk assessment
Soil
virus
Viruses
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aims The aim of this study was to determine how the transfer efficiency of MS‐2 coliphage from the toilet seat to hands and fingertip to lip differs according to the suspension of the inoculum. Methods and Results Hands were sampled after lifting a toilet seat which was inoculated with MS‐2 on the underneath side. MS‐2 was suspended in a spectrum of proteinaceous and non‐proteinaceous solutions. Transfer efficiencies were greatest with the ASTM tripartite soil load (3.02% ± 4.03) and lowest with phosphate‐buffered saline (PBS) (1.10% ± 0.81) for hand‐to‐toilet seat contacts. Finger‐to‐lip transfer rates were significantly different (p 
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.15758