Oxidative lesions and post‐treatment viability attenuation of listeria monocytogenes triggered by atmospheric non‐thermal plasma

Aims The aim of the current study was to investigate the effect of plasma‐mediated oxidative stress on the post‐treatment viability of Listeria monocytogenes at the physiological and molecular levels. Methods and Results 107 CFU/ml L. monocytogenes in 10 ml phosphate‐buffered saline (PBS) was treate...

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Bibliographic Details
Published in:Journal of applied microbiology 2022-10, Vol.133 (4), p.2348-2360
Main Authors: Pan, Yuanyuan, Cheng, Jun‐Hu, Sun, Da‐Wen
Format: Article
Language:English
Subjects:
Acidification
Attenuation
DBD plasma
Deactivation
Deoxyribonucleic acid
Dielectric barrier discharge
DNA
DNA - metabolism
DNA fragmentation
DNA lesions
DNA repair
Emission spectroscopy
Energy metabolism
Food
Foodborne pathogens
gene expression
Genes
L. Monocytogenes
Listeria
Listeria monocytogenes
Nitrogen - metabolism
Optical emission spectroscopy
Original
Oxidation resistance
Oxidative Stress
Oxygen - metabolism
Oxygen consumption
Phosphates - metabolism
Plasma
Reactive nitrogen species
Reactive oxygen species
Reactive Oxygen Species - metabolism
RecA protein
Resuscitation
Spectroscopy
Thermal plasmas
Viability
Virulence
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Summary:Aims The aim of the current study was to investigate the effect of plasma‐mediated oxidative stress on the post‐treatment viability of Listeria monocytogenes at the physiological and molecular levels. Methods and Results 107 CFU/ml L. monocytogenes in 10 ml phosphate‐buffered saline (PBS) was treated with atmospheric non‐thermal plasma for 0, 30, 60, 90 and 120 s respectively. Optical diagnostics using optical emission spectroscopy (OES) confirmed that dielectric barrier discharge (DBD) plasma was a significant source of ample exogenous reactive oxygen and nitrogen species (RONS). The development of extracellular main long‐lived species was associated with plasma exposure time, accompanied by a massive accumulation of intracellular ROS in L. monocytogenes (p 
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.15688