Tomography of DNA tiles influences the kinetics of surface-mediated DNA self-assembly

This manuscript studies the impact of extruding hairpins on two-dimensional self-assembly of DNA tiles on solid surface. Hairpins are commonly used as tomographic markers in DNA nanostructures for atomic force microscopy imaging. In this study, we have discovered that hairpins play a more active rol...

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Bibliographic Details
Published in:Biophysical journal 2022-12, Vol.121 (24), p.4909-4914
Main Authors: Zhang, Cuizheng, Paluzzi, Victoria E., Mao, Chengde
Format: Article
Language:English
Subjects:
DNA - chemistry
Kinetics
Microscopy, Atomic Force
Nanostructures - chemistry
Nanotechnology - methods
Nucleic Acid Conformation
Tomography
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Summary:This manuscript studies the impact of extruding hairpins on two-dimensional self-assembly of DNA tiles on solid surface. Hairpins are commonly used as tomographic markers in DNA nanostructures for atomic force microscopy imaging. In this study, we have discovered that hairpins play a more active role. They modulate the adsorption of the DNA tiles onto the solid surface, thus changing the tile assembly kinetics on the solid surface. Based on this discovery, we were able to promote or slow down DNA self-assembly on the surface by changing the hairpin locations on the DNA tiles. This knowledge gained will be helpful for the future design of DNA self-assembly on surface. [Display omitted]
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2022.07.025