Conditioning Regimens are Associated with Distinct Patterns of Microbiota Injury in Allogeneic Hematopoietic Cell Transplantation

• Published in: Clinical cancer research 2023-01, Vol.29 (1), p.165-173
• Main Authors: Shouval, Roni, Waters, Nicholas R, Gomes, Antonio L C, Zuanelli Brambilla, Corrado, Fei, Teng, Devlin, Sean M, Nguyen, Chi L, Markey, Kate A, Dai, Anqi, Slingerland, John B, Clurman, Annelie G, Fontana, Emily, Amoretti, Luigi A, Wright, Roberta J, Hohl, Tobias M, Taur, Ying, Sung, Anthony D, Weber, Daniela, Hashimoto, Daigo, Teshima, Takanori, Chao, Nelson J, Holler, Ernst, Scordo, Michael, Giralt, Sergio A, Perales, Miguel-Angel, Peled, Jonathan U, van den Brink, Marcel R M
• Format: Article
• Language: English
• Subjects: Cyclophosphamide - adverse effects; Graft vs Host Disease - diagnosis; Graft vs Host Disease - etiology; Hematopoietic Stem Cell Transplantation - adverse effects; Humans; Microbiota; Retrospective Studies; RNA, Ribosomal, 16S; Transplantation Conditioning - adverse effects; Transplantation, Homologous - adverse effects
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.CCR-22-1254

The gut microbiota is subject to multiple insults in allogeneic hematopoietic cell transplantation (allo-HCT) recipients. We hypothesized that preparative conditioning regimens contribute to microbiota perturbation in allo-HCT. This was a retrospective study that evaluated the relationship between conditioning regimens exposure in 1,188 allo-HCT recipients and the gut microbiome. Stool samples collected from 20 days before transplantation up to 30 days after were profiled using 16S rRNA sequencing. Microbiota injury was quantified by changes in α-diversity. We identified distinct patterns of microbiota injury that varied by conditioning regimen. Diversity loss was graded into three levels of conditioning-associated microbiota injury (CMBI) in a multivariable model that included antibiotic exposures. High-intensity regimens, such as total body irradiation (TBI)-thiotepa-cyclophosphamide, were associated with the greatest injury (CMBI III). In contrast, the nonmyeloablative regimen fludarabine-cyclophosphamide with low-dose TBI (Flu/Cy/TBI200) had a low-grade injury (CMBI I). The risk of acute GVHD correlated with CMBI degree. Pretransplant microbial compositions were best preserved with Flu/Cy/TBI200, whereas other regimens were associated with loss of commensal bacteria and expansion of Enterococcus. Our findings support an interaction between conditioning at the regimen level and the extent of microbiota injury.