Novel detection method to rapidly quantify toxic cucurbitacin in Lagenaria siceraria (bottle gourd)

Bottle gourd ( Lagenaria siceraria ) is widely used in India for its medicinal properties. Sometimes its consumption as a fruit or juice can lead to serious health issues due to the presence of toxic cucurbitacins (Cuc) like Cuc B at high concentration. In the present study, a molecularly imprinted...

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Bibliographic Details
Published in:Journal of food science and technology 2023-01, Vol.60 (1), p.160-170
Main Authors: Saurabh, Chaturbhuj Kumar, Ghosh, Sunil Kumar, Sanyal, Bhaskar
Format: Article
Language:English
Subjects:
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Cucurbita
Food Science
Fruits
Gamma irradiation
Glucosidase
Gourds
Imprinted polymers
Irradiation
Juices
Lagenaria siceraria
Nutrition
Original Paper
Polymers
Pulp
Quality assurance
Scatchard curves
Selectivity
Solid phases
β-Glucosidase
γ Radiation
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Summary:Bottle gourd ( Lagenaria siceraria ) is widely used in India for its medicinal properties. Sometimes its consumption as a fruit or juice can lead to serious health issues due to the presence of toxic cucurbitacins (Cuc) like Cuc B at high concentration. In the present study, a molecularly imprinted polymer (MIP) based method was developed to quantify Cuc B in commercial bottle gourd juice. Cuc B quantification was also done in peel and pulp of fresh bottle gourd fruit. Developed MIP was superior to NIP (non-imprinted polymer) in terms of equilibrium binding, solid-phase extraction, selectivity, and loading capacity of Cuc B. Scatchard plot analysis, adsorption kinetics, and surface area study further strengthen the fact that prepared imprinted polymer was better than NIP for Cuc extraction. TLC and high-resolution LC–MS based analysis revealed that MIP selectively extracts Cuc B from all the studied samples. It was further observed that gamma irradiation and β -glucosidase treatment did not have any significant ( p  > 0.05) effect on Cuc concentration. The quantity of Cuc in 100 g of juice, peel, and pulp was 2.81 ± 0.26, 3.37 ± 0.31, and 2.39 ± 0.21 mg, respectively. Analytical method validation indicates that the MIP based method was highly linear, precise, and accurate to rapidly quantify toxic Cuc B in bottle gourd consequently assuring its quality.
ISSN:0022-1155
0975-8402
DOI:10.1007/s13197-022-05600-3