A Chloroplast-Localized Glucose-6-Phosphate Dehydrogenase Positively Regulates Stripe Rust Resistance in Wheat

Glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme of the pentose phosphate pathway (PPP), plays a pivotal role in plant stress responses. However, the function and mechanism of G6PDHs in crop plants challenged by fungal pathogens remain poorly understood. In this study, a wheat G6D...

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Published in:International journal of molecular sciences 2022-12, Vol.24 (1), p.459
Main Authors: Wei, Xiaobo, Huang, Xueling, Yang, Weiling, Wang, Xinran, Guan, Tao, Kang, Zhensheng, Liu, Jie
Format: Article
Language:English
Subjects:
Basidiomycota - genetics
Disease Resistance - genetics
Gene Silencing
Glucosephosphate Dehydrogenase - genetics
Glucosephosphate Dehydrogenase - metabolism
Hydrogen Peroxide - metabolism
Plant Diseases - genetics
Plant Diseases - microbiology
Triticum - metabolism
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Summary:Glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme of the pentose phosphate pathway (PPP), plays a pivotal role in plant stress responses. However, the function and mechanism of G6PDHs in crop plants challenged by fungal pathogens remain poorly understood. In this study, a wheat G6DPH gene responding to infection by f. sp. ( ), designated , was cloned and functionally identified. expression was significantly upregulated in wheat leaves inoculated with or treated with abiotic stress factors. Heterologous mutant complementation and enzymatic properties indicate that encodes a G6PDH protein. The transient expression of in leaves and wheat protoplasts revealed that TaG6PDH2 is a chloroplast-targeting protein. Silencing via the barley stripe mosaic virus (BSMV)-induced gene silencing (VIGS) system led to compromised wheat resistance to the avirulent pathotype CYR23, which is implicated in weakened H O accumulation and cell death. In addition, TaG6PDH2 was confirmed to interact with the wheat glutaredoxin TaGrxS4. These results demonstrate that endows wheat with increased resistance to stripe rust by regulating reactive oxygen species (ROS) production.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms24010459