Design and Investigation of a Photocatalytic Setup for Efficient Biotransformations Within Recombinant Cyanobacteria in Continuous Flow

Photo‐ and biocatalysis show many advantages as more sustainable solutions for the production of fine chemicals. In an effort to combine the benefits and the knowledge of both these areas, a continuous photobiocatalytic setup was designed and optimized to carry out whole‐cell biotransformations with...

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Bibliographic Details
Published in:ChemSusChem 2022-11, Vol.15 (22), p.e202201468-n/a
Main Authors: Valotta, Alessia, Malihan‐Yap, Lenny, Hinteregger, Kerstin, Kourist, Robert, Gruber‐Woelfler, Heidrun
Format: Article
Language:English
Subjects:
Biocatalysis
Biotransformation
Coils
Continuous flow
Cyanobacteria
Fine chemicals
Flow velocity
Light
Luminous intensity
Oxidoreductases - metabolism
photobiocatalysis
Photosynthesis
Reductases
Stereoselectivity
Synechocystis - genetics
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Summary:Photo‐ and biocatalysis show many advantages as more sustainable solutions for the production of fine chemicals. In an effort to combine the benefits and the knowledge of both these areas, a continuous photobiocatalytic setup was designed and optimized to carry out whole‐cell biotransformations within cells of the cyanobacterium Synechocystis sp. PCC 6803 expressing the gene of the ene‐reductase YqjM from B. subtilis. The effect of the light intensity and flow rate on the specific activity in the stereoselective reduction of 2‐methyl maleimide was investigated via a design‐of‐experiments approach. The cell density in the setup was further increased at the optimal operating conditions without loss in specific activity, demonstrating that the higher surface area/volume ratio in the coil reactor improved the illumination efficiency of the process. Furthermore, different reactor designs were compared, proving that the presented approach was the most cost‐ and time‐effective solution for intensifying photobiotransformations within cyanobacterial cells. Green flow with glow: In this work, the benefits of flow photo‐ and biocatalysis are combined in a continuous setup for ene‐reduction reactions within recombinant cyanobacteria. The designed setup, comprising a helical coil reactor, allows for a very high surface area/volume ratio (≈2000 m2 m−3) and improved light distribution, thereby limiting the self‐shadowing of the cells and allowing for improved productivity compared to batch reactors.
ISSN:1864-5631
1864-564X
DOI:10.1002/cssc.202201468