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Identification of novel proteins involved in P2X7-mediated signaling cascades
High concentration of extracellular ATP acts as a danger signal that is sensed by the P2X7 receptor (P2X7R). This ATP-gated ion channel has been shown to induce multiple metabotropic events such as changes in plasma membrane composition and morphology, ectodomain shedding, activation of lipases, kin...
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Published in: | Purinergic signalling 2022-12, Vol.18 (4), p.495-498 |
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container_title | Purinergic signalling |
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description | High concentration of extracellular ATP acts as a danger signal that is sensed by the P2X7 receptor (P2X7R). This ATP-gated ion channel has been shown to induce multiple metabotropic events such as changes in plasma membrane composition and morphology, ectodomain shedding, activation of lipases, kinases, and transcription factors as well as cytokine release. The specific signaling pathways and molecular mechanisms remain largely obscure. Using an unbiased genome-scale CRISPR/Cas9 screening approach in a murine T cell line, Ryoden et al. (2022, 2020) identified three proteins involved in P2X7 regulation and signaling: Essential for Reactive Oxygen Species (EROS) is essential for P2X7 folding and maturation, and Xk and Vsp13a are required for P2X7-mediated phosphatidyl serine exposure and cell lysis. They further provide evidence for an interaction of Xk and Vsp13a at the plasma membrane and confirm the role of Xk in ATP-induced cytolysis in primary CD25
+
CD4
+
T cells from Xk
−/−
mice. |
doi_str_mv | 10.1007/s11302-022-09893-z |
format | article |
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+
CD4
+
T cells from Xk
−/−
mice.</description><identifier>ISSN: 1573-9538</identifier><identifier>EISSN: 1573-9546</identifier><identifier>DOI: 10.1007/s11302-022-09893-z</identifier><identifier>PMID: 35960424</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adenosine Triphosphate - pharmacology ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; CD25 antigen ; CD4 antigen ; CRISPR ; Cytolysis ; Genomes ; Human Physiology ; Journal Club ; Lymphocyte Activation ; Lymphocytes T ; Lysis ; Membrane composition ; Metabotropic receptors ; Mice ; Molecular modelling ; Neurosciences ; Pharmacology/Toxicology ; Reactive oxygen species ; Receptors, Purinergic P2X7 ; Signal Transduction ; T-Lymphocytes ; Transcription factors</subject><ispartof>Purinergic signalling, 2022-12, Vol.18 (4), p.495-498</ispartof><rights>The Author(s) 2022</rights><rights>2022. The Author(s).</rights><rights>The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-8988dd8fac7cb8a76d3e597ba3e60a316950ec92bcb236b184f3d011193dc0b93</citedby><cites>FETCH-LOGICAL-c474t-8988dd8fac7cb8a76d3e597ba3e60a316950ec92bcb236b184f3d011193dc0b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832184/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832184/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35960424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sassenbach, Lukas</creatorcontrib><title>Identification of novel proteins involved in P2X7-mediated signaling cascades</title><title>Purinergic signalling</title><addtitle>Purinergic Signalling</addtitle><addtitle>Purinergic Signal</addtitle><description>High concentration of extracellular ATP acts as a danger signal that is sensed by the P2X7 receptor (P2X7R). This ATP-gated ion channel has been shown to induce multiple metabotropic events such as changes in plasma membrane composition and morphology, ectodomain shedding, activation of lipases, kinases, and transcription factors as well as cytokine release. The specific signaling pathways and molecular mechanisms remain largely obscure. Using an unbiased genome-scale CRISPR/Cas9 screening approach in a murine T cell line, Ryoden et al. (2022, 2020) identified three proteins involved in P2X7 regulation and signaling: Essential for Reactive Oxygen Species (EROS) is essential for P2X7 folding and maturation, and Xk and Vsp13a are required for P2X7-mediated phosphatidyl serine exposure and cell lysis. They further provide evidence for an interaction of Xk and Vsp13a at the plasma membrane and confirm the role of Xk in ATP-induced cytolysis in primary CD25
+
CD4
+
T cells from Xk
−/−
mice.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>CD25 antigen</subject><subject>CD4 antigen</subject><subject>CRISPR</subject><subject>Cytolysis</subject><subject>Genomes</subject><subject>Human Physiology</subject><subject>Journal Club</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes T</subject><subject>Lysis</subject><subject>Membrane composition</subject><subject>Metabotropic receptors</subject><subject>Mice</subject><subject>Molecular modelling</subject><subject>Neurosciences</subject><subject>Pharmacology/Toxicology</subject><subject>Reactive oxygen species</subject><subject>Receptors, Purinergic P2X7</subject><subject>Signal Transduction</subject><subject>T-Lymphocytes</subject><subject>Transcription factors</subject><issn>1573-9538</issn><issn>1573-9546</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kUtLAzEQx4Motj6-gAdZ8Lyaxz6SiyDFR6GiBwVvIZtk15RtUpPtgv30RrdWvXgIGTL_-c1M_gCcIHiOICwvAkIE4hTieBhlJF3vgDHKS5KyPCt2tzGhI3AQwhzCPMOE7YMRyVkBM5yNwf1UaduZ2kjRGWcTVyfW9bpNlt512tiQGNu7ttcqBskjfinThVZGdPEhmMaK1tgmkSJIoXQ4Anu1aIM-3tyH4Pnm-mlyl84ebqeTq1kqszLrUsooVYrWQpayoqIsFNE5KytBdAEFQQXLoZYMV7LCpKgQzWqiIEKIESVhxcghuBy4y1UVx5FxBS9avvRmIfw7d8LwvxlrXnnjes4owREXAWcbgHdvKx06PncrH7cJHJcFyXJEMYwqPKikdyF4XW87IMg_LeCDBTxawL8s4OtYdPp7tm3J959HARkEIaZso_1P73-wH302k54</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Sassenbach, Lukas</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20221201</creationdate><title>Identification of novel proteins involved in P2X7-mediated signaling cascades</title><author>Sassenbach, Lukas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-8988dd8fac7cb8a76d3e597ba3e60a316950ec92bcb236b184f3d011193dc0b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>CD25 antigen</topic><topic>CD4 antigen</topic><topic>CRISPR</topic><topic>Cytolysis</topic><topic>Genomes</topic><topic>Human Physiology</topic><topic>Journal Club</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes T</topic><topic>Lysis</topic><topic>Membrane composition</topic><topic>Metabotropic receptors</topic><topic>Mice</topic><topic>Molecular modelling</topic><topic>Neurosciences</topic><topic>Pharmacology/Toxicology</topic><topic>Reactive oxygen species</topic><topic>Receptors, Purinergic P2X7</topic><topic>Signal Transduction</topic><topic>T-Lymphocytes</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sassenbach, Lukas</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Purinergic signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sassenbach, Lukas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of novel proteins involved in P2X7-mediated signaling cascades</atitle><jtitle>Purinergic signalling</jtitle><stitle>Purinergic Signalling</stitle><addtitle>Purinergic Signal</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>18</volume><issue>4</issue><spage>495</spage><epage>498</epage><pages>495-498</pages><issn>1573-9538</issn><eissn>1573-9546</eissn><abstract>High concentration of extracellular ATP acts as a danger signal that is sensed by the P2X7 receptor (P2X7R). This ATP-gated ion channel has been shown to induce multiple metabotropic events such as changes in plasma membrane composition and morphology, ectodomain shedding, activation of lipases, kinases, and transcription factors as well as cytokine release. The specific signaling pathways and molecular mechanisms remain largely obscure. Using an unbiased genome-scale CRISPR/Cas9 screening approach in a murine T cell line, Ryoden et al. (2022, 2020) identified three proteins involved in P2X7 regulation and signaling: Essential for Reactive Oxygen Species (EROS) is essential for P2X7 folding and maturation, and Xk and Vsp13a are required for P2X7-mediated phosphatidyl serine exposure and cell lysis. They further provide evidence for an interaction of Xk and Vsp13a at the plasma membrane and confirm the role of Xk in ATP-induced cytolysis in primary CD25
+
CD4
+
T cells from Xk
−/−
mice.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>35960424</pmid><doi>10.1007/s11302-022-09893-z</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adenosine Triphosphate - pharmacology Animals Biomedical and Life Sciences Biomedicine Cancer Research CD25 antigen CD4 antigen CRISPR Cytolysis Genomes Human Physiology Journal Club Lymphocyte Activation Lymphocytes T Lysis Membrane composition Metabotropic receptors Mice Molecular modelling Neurosciences Pharmacology/Toxicology Reactive oxygen species Receptors, Purinergic P2X7 Signal Transduction T-Lymphocytes Transcription factors |
title | Identification of novel proteins involved in P2X7-mediated signaling cascades |
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