Silencing TRAIP suppresses cell proliferation and migration/invasion of triple negative breast cancer via RB-E2F signaling and EMT

• Published in: Cancer gene therapy 2023-01, Vol.30 (1), p.74-84
• Main Authors: Zheng, Yan, Jia, Huiqing, Wang, Ping, Liu, Litong, Chen, Zhaoxv, Xing, Xiaoming, Wang, Jin, Tan, Xiaohua, Wang, Chengqin
• Format: Article
• Language: English
• Subjects: 13/1; 13/109; 13/31; 3' Untranslated regions; 38/44; 38/79; 42/41; 631/208/199; 631/67/1347; 631/67/322; 64/60; 692/308/53; 692/699/67/395; 82/80; Animal models; Animal research; Animals; Biomedical and Life Sciences; Biomedicine; Breast cancer; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; Cholecystokinin; E2F protein; Epithelial-Mesenchymal Transition - genetics; Flow cytometry; Gene Expression; Gene Expression Regulation, Neoplastic; Gene Therapy; Humans; Immunohistochemistry; Kinases; Mesenchyme; Metastases; Mice; MicroRNAs - genetics; MicroRNAs - metabolism; Therapeutic targets; Triple Negative Breast Neoplasms - metabolism; Tumorigenesis; Ubiquitin; Ubiquitin-protein ligase; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Wound healing; Xenografts
• ISSN: 0929-1903; 1476-5500
• DOI: 10.1038/s41417-022-00517-7

TRAIP, as a 53 kDa E3 ubiquitin protein ligase, is involved in various cellular processes and closely related to the occurrence and development of tumors. At present, few studies on the relationship between TRAIP and triple negative breast cancer (TNBC) were reported. Bioinformatic analysis and Western blot, immunohistochemistry (IHC), CCK-8, colony formation, flow cytometry, wound healing, Transwell, and dual-luciferase reporter assays were performed, and xenograft mouse models were established to explore the role of TRAIP in TNBC. This study showed that the expression of TRAIP protein was upregulated in TNBC tissues and cell lines. Silencing of TRAIP significantly inhibited the proliferation, migration, and invasion of TNBC cells, whereas opposite results were observed in the TRAIP overexpression. In addition, TRAIP regulated cell proliferation, migration, and invasion through RB-E2F signaling and epithelial mesenchymal transformation (EMT). MiR-590-3p directly targeted the TRAIP 3′-UTR, and its expression were lower in TNBC tissues. Its mimic significantly downregulated the expression of TRAIP and subsequently suppressed cell proliferation, migration, and invasion. Rescue experiments indicated that TRAIP silencing reversed the promotion of miR-590-3p inhibitor on cell proliferation, migration, and invasion. TRAIP overexpression could also reverse the inhibition of miR-590-3p mimic on tumorigenesis. Finally, TRAIP knockdown significantly inhibited tumor growth and metastasis in animal experiments. In conclusion, TRAIP is an oncogene that influences the proliferation, migration, and invasion of TNBC cells through RB-E2F signaling and EMT. Therefore, TRAIP may be a potential therapeutic target for TNBC.