Nicorandil protects podocytes via modulation of antioxidative capacity in acute puromycin aminonucleoside-induced nephrosis in rats

• Published in: American journal of physiology. Renal physiology 2023-02, Vol.324 (2), p.F168-F178
• Main Authors: Yamanaka, Masaki, Tamura, Yoshifuru, Kuribayashi-Okuma, Emiko, Uchida, Shunya, Shibata, Shigeru
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - metabolism; Animals; Antioxidants - metabolism; Calcium (mitochondrial); Deoxyguanosine; Electron microscopy; End-stage renal disease; Hypoalbuminemia; Immunosuppressive agents; Kidney diseases; Manganese; Mitochondria; Nephrosis - chemically induced; Nephrosis - prevention & control; Nephrotic syndrome; Nephrotic Syndrome - chemically induced; Nephrotic Syndrome - drug therapy; Nephrotic Syndrome - prevention & control; Nicorandil - therapeutic use; Nitrotyrosine; Oral administration; Oxidative stress; Podocytes; Potassium channels; Proteinuria; Proteinuria - chemically induced; Proteinuria - prevention & control; Puromycin; Puromycin Aminonucleoside - toxicity; Rats; Superoxide Dismutase
• ISSN: 1931-857X; 1522-1466
• DOI: 10.1152/ajprenal.00144.2022

Nephrotic syndrome, characterized by proteinuria and hypoalbuminemia, results from the dysregulation of glomerular podocytes and is a significant cause of end-stage kidney disease. Patients with idiopathic nephrotic syndrome are generally treated with immunosuppressive agents; however, these agents produce various adverse effects. Previously, we reported the renoprotective effects of a stimulator of the mitochondrial ATP-dependent K channel (MitK ), nicorandil, in a remnant kidney model. Nonetheless, the cellular targets of these effects remain unknown. Here, we examined the effect of nicorandil on puromycin aminonucleoside-induced nephrosis (PAN) rats, a well-established model of podocyte injury and human nephrotic syndrome. PAN was induced using a single intraperitoneal injection. Nicorandil was administered orally at 30 mg/kg/day. We found that proteinuria and hypoalbuminemia in PAN rats were significantly ameliorated following nicorandil treatment. Immunostaining and ultrastructural analysis under electron microscopy demonstrated that podocyte injury in PAN rats showed a significant partial attenuation following nicorandil treatment. Nicorandil ameliorated the increase in the oxidative stress markers nitrotyrosine and 8-hydroxy-2-deoxyguanosine in glomeruli. Conversely, nicorandil prevented the decrease in levels of the antioxidant enzyme manganese superoxide dismutase in PAN rats. We found that mitochondrial Ca uniporter levels in glomeruli were higher in PAN rats than in control rats, and this increase was significantly attenuated by nicorandil. We conclude that stimulation of MitK by nicorandil reduces proteinuria by attenuating podocyte injury in PAN nephrosis, which restores mitochondrial antioxidative capacity, possibly through mitochondrial Ca uniporter modulation. These data indicate that MitK may represent a novel target for podocyte injury and nephrotic syndrome. Our findings suggest that the mitochondrial Ca uniporter may be an upstream regulator of manganese superoxide dismutase and indicate a biochemical basis for the interaction between the ATP-sensitive K channel and Ca signaling. We believe that our study makes a significant contribution to the literature because our results indicate that the ATP-sensitive K channel may be a potential therapeutic target for podocyte injury and nephrotic syndrome.