The Impact of Tissue Preparation on Salivary Gland Tumors Investigated by Fourier-Transform Infrared Microspectroscopy

Due to the wide variety of benign and malignant salivary gland tumors, classification and malignant behavior determination based on histomorphological criteria can be difficult and sometimes impossible. Spectroscopical procedures can acquire molecular biological information without destroying the ti...

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Bibliographic Details
Published in:Journal of clinical medicine 2023-01, Vol.12 (2), p.569
Main Authors: Stefanakis, Mona, Bassler, Miriam C, Walczuch, Tobias R, Gerhard-Hartmann, Elena, Youssef, Almoatazbellah, Scherzad, Agmal, Stöth, Manuel Bernd, Ostertag, Edwin, Hagen, Rudolf, Steinke, Maria R, Hackenberg, Stephan, Brecht, Marc, Meyer, Till Jasper
Format: Article
Language:English
Subjects:
Clinical medicine
Data analysis
Discriminant analysis
Exocrine glands
Investigations
Mercury cadmium telluride
Oral cancer
Spectrum analysis
Tumors
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Summary:Due to the wide variety of benign and malignant salivary gland tumors, classification and malignant behavior determination based on histomorphological criteria can be difficult and sometimes impossible. Spectroscopical procedures can acquire molecular biological information without destroying the tissue within the measurement processes. Since several tissue preparation procedures exist, our study investigated the impact of these preparations on the chemical composition of healthy and tumorous salivary gland tissue by Fourier-transform infrared (FTIR) microspectroscopy. Sequential tissue cross-sections were prepared from native, formalin-fixed and formalin-fixed paraffin-embedded (FFPE) tissue and analyzed. The FFPE cross-sections were dewaxed and remeasured. By using principal component analysis (PCA) combined with a discriminant analysis (DA), robust models for the distinction of sample preparations were built individually for each parotid tissue type. As a result, the PCA-DA model evaluation showed a high similarity between native and formalin-fixed tissues based on their chemical composition. Thus, formalin-fixed tissues are highly representative of the native samples and facilitate a transfer from scientific laboratory analysis into the clinical routine due to their robust nature. Furthermore, the dewaxing of the cross-sections entails the loss of molecular information. Our study successfully demonstrated how FTIR microspectroscopy can be used as a powerful tool within existing clinical workflows.
ISSN:2077-0383
2077-0383
DOI:10.3390/jcm12020569