Cloning, expression, and catalytic activity of Helicobacter hepaticus urease

Helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. It is strongly urease positive, although it does not live in an acidic environment. The H. hepaticus urease gene cluster was expressed in Escherichia coli with and without coexpression of the Helicobacter pylori n...

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Bibliographic Details
Published in:Infection and immunity 2001-09, Vol.69 (9), p.5914-5920
Main Authors: BECKWITH, Catherine S, MCGEE, David J, MOBLEY, Harry L. T, RILEY, Lela K
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bacteriology
Biological and medical sciences
Cloning, Molecular
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetics
Helicobacter - enzymology
Helicobacter - genetics
Helicobacter hepaticus
Metabolism. Enzymes
Mice
Microbiology
Molecular Pathogenesis
Molecular Sequence Data
Multigene Family
nik gene
nik genes
Sequence Analysis, DNA
Urease - chemistry
Urease - genetics
Urease - metabolism
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Summary:Helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. It is strongly urease positive, although it does not live in an acidic environment. The H. hepaticus urease gene cluster was expressed in Escherichia coli with and without coexpression of the Helicobacter pylori nickel transporter NixA. As for H. pylori, it was difficult to obtain enzymatic activity from recombinant H. hepaticus urease; special conditions including NiCl2 supplementation were required. The H. hepaticus urease cluster contains a homolog of each gene in the H. pylori urease cluster, including the urea transporter gene ureI. Downstream genes were homologs of the nik nickel transport operon of E. coli. Nongastric H. hepaticus produces urease similar to that of H. pylori.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.69.9.5914-5920.2001