Numerical refractive index correction for the stitching procedure in tomographic quantitative phase imaging

Tomographic quantitative phase imaging (QPI) lacks an absolute refractive index value baseline, which poses a problem when large dense objects extending over multiple fields of view are measured volume by volume and stitched together. Some of the measurements lack the natural baseline value that is...

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Bibliographic Details
Published in:Biomedical optics express 2022-11, Vol.13 (11), p.5709-5720
Main Authors: Stępień, Piotr, Ziemczonok, Michał, Kujawińska, Małgorzata, Baczewska, Maria, Valenti, Luca, Cherubini, Alessandro, Casirati, Elia, Krauze, Wojciech
Format: Article
Language:English
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Summary:Tomographic quantitative phase imaging (QPI) lacks an absolute refractive index value baseline, which poses a problem when large dense objects extending over multiple fields of view are measured volume by volume and stitched together. Some of the measurements lack the natural baseline value that is provided by the mounting medium with a known refractive index. In this work, we discuss the problem of the refractive index (RI) baseline of individual reconstructed volumes that are deprived of access to mounting medium due to the extent of the object. The solution of this problem is provided by establishing the RI offsets based on the overlapping regions. We have proven that the process of finding the offset RI values may be justifiably reduced to the analogous procedure in the 2D baseline correction (2D-BC). Finally, we proposed the enhancement of the state-of-the-art 2D-BC procedure previously introduced in the context of 2D QPI. The processing is validated at the examples of a synthetic dataset and a liver organoid.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.466403