Molecular characterization of eliminated chromosomes in Hessian fly (Mayetiola destructor (Say))

Like other cecidomyiid Diptera, Hessian fly has stable S chromosomes and dispensable E chromosomes that are retained only in the germ line. Amplified fragment length polymorphisms (AFLP), suppressive subtractive hybridization (SSH), fluorescent in-situ hybridization (FISH), and sequencing were used...

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Bibliographic Details
Published in:Chromosome research 2023-01, Vol.31 (1), p.3, Article 3
Main Authors: Crane, Yan M., Crane, Charles F., Cambron, Sue E., Springmeyer, Lucy J., Schemerhorn, Brandon J.
Format: Article
Language:English
Subjects:
Amplified fragment length polymorphism
Amplified Fragment Length Polymorphism Analysis
Animal Genetics and Genomics
Animals
Base Sequence
Biomedical and Life Sciences
Cell Biology
Chromosomes
Diptera - genetics
DNA - genetics
Female
Human Genetics
Hybridization
Life Sciences
Mayetiola destructor
Ovaries
Plant Genetics and Genomics
Sequence analysis
Sex chromosomes
Sex Chromosomes - genetics
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Summary:Like other cecidomyiid Diptera, Hessian fly has stable S chromosomes and dispensable E chromosomes that are retained only in the germ line. Amplified fragment length polymorphisms (AFLP), suppressive subtractive hybridization (SSH), fluorescent in-situ hybridization (FISH), and sequencing were used to investigate similarities and differences between S and E chromosomes. More than 99.9% of AFLP bands were identical between separated ovary and somatic tissue, but one band was unique to ovary and resembled Worf , a non-LTR retrotransposon. Arrayed clones, derived by SSH of somatic from ovarian DNA, showed no clones that were unique to ovary. FISH with BAC clones revealed a diagnostic banding pattern of BAC positions on both autosomes and both sex chromosomes, and each E chromosome shared a pattern with one of the S chromosomes. Sequencing analysis showed that E chromosomes are nearly identical to S chromosomes, since no sequence could be confirmed to belong only to E chromosomes. There were a few questionably E-specific sequences that are candidates for further investigation. Thus, the E chromosomes appear to be derived from S chromosomes by the acquisition or conversion of sequences that produce the negatively heteropycnotic region around the centromere.
ISSN:1573-6849
0967-3849
1573-6849
DOI:10.1007/s10577-023-09718-8