Anti-inflammatory, Antinociceptive, and Toxicological Properties of Uvaria comperei Stem Crude Extract and Fractions

• Published in: BioMed research international 2023, Vol.2023 (1), p.2754725-2754725
• Main Authors: Simo, Marguerite Kamdem, Siwe, Gael Tchokomeni, Taboula Kayo, Maurice, Chen, Zheng, Mangoua Kouamo, Mersimine, Dize, Darline, Jazet, Pierre Dongmo, Sameza, Modeste Lambert, Fekam, Fabrice Boyom, Froldi, Guglielmina
• Format: Article
• Language: English
• Subjects: Acute toxicity; Albumin; Albumins; Analgesics; Animals; Anti-inflammatory agents; Anti-Inflammatory Agents - therapeutic use; Anti-inflammatory drugs; Biocompatibility; Blood; Carrageenan; Carrageenan - adverse effects; Carrageenans; Chemical properties; Chemical research; Cytotoxicity; Denaturation; Edema; Edema - chemically induced; Edema - drug therapy; Enzymes; Erythrocytes; Flavonoids; Formaldehyde; Health aspects; High performance liquid chromatography; Inflammation; Inhibition; Laboratory animals; Liquid chromatography; Lysis; Magnoliidae; Materia medica, Vegetable; Metabolites; Methanol; Mice; Nonsteroidal anti-inflammatory drugs; Pain; Pain - drug therapy; Pain perception; Plant extracts; Plant Extracts - chemistry; Stems; Toxicity; Uvaria
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2023/2754725

The present study was carried out to investigate the anti-inflammatory activity of a methanolic extract and fractions of Uvaria comperei stems. The crude extract was obtained by maceration of the powder in methanol and fractions by vacuum chromatography from the methanolic extract. To study the anti-inflammatory activity in vitro, red blood cell lysis inhibition assay and albumin denaturation inhibition were performed, while in vivo measurements of carrageenan-induced paw oedema and formalin-induced pain in albino mice were performed. Acute toxicity and cytotoxicity studies of the fraction F2 were performed, as well as its HPLC, and some biochemical parameters were quantified. Uvaria comperei crude extract (UCCE) at 250 and 500 μg/mL completely inhibited albumin denaturation, while decreasing 75.5% of heat blood cell lysis at 500 μg/mL. The fractions 128-136 (F3), 10-11 (F1), and 56-62 (F2) at 500 μg/mL displayed a significant anti-inflammatory activity with percentages of inhibition of 60.5, 67.4, and 100%, respectively. Administration of fraction F2 (25, 50, and 100 mg/kg, p.o.) produced a dose-dependent inhibition of formalin-induced pain of 60.2% at 50 mg/kg in the neurogenic phase (p