Anti-inflammatory, Antinociceptive, and Toxicological Properties of Uvaria comperei Stem Crude Extract and Fractions

The present study was carried out to investigate the anti-inflammatory activity of a methanolic extract and fractions of Uvaria comperei stems. The crude extract was obtained by maceration of the powder in methanol and fractions by vacuum chromatography from the methanolic extract. To study the anti...

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Published in:BioMed research international 2023, Vol.2023 (1), p.2754725-2754725
Main Authors: Simo, Marguerite Kamdem, Siwe, Gael Tchokomeni, Taboula Kayo, Maurice, Chen, Zheng, Mangoua Kouamo, Mersimine, Dize, Darline, Jazet, Pierre Dongmo, Sameza, Modeste Lambert, Fekam, Fabrice Boyom, Froldi, Guglielmina
Format: Article
Language:English
Subjects:
Acute toxicity
Albumin
Albumins
Analgesics
Animals
Anti-inflammatory agents
Anti-Inflammatory Agents - therapeutic use
Anti-inflammatory drugs
Biocompatibility
Blood
Carrageenan
Carrageenan - adverse effects
Carrageenans
Chemical properties
Chemical research
Cytotoxicity
Denaturation
Edema
Edema - chemically induced
Edema - drug therapy
Enzymes
Erythrocytes
Flavonoids
Formaldehyde
Health aspects
High performance liquid chromatography
Inflammation
Inhibition
Laboratory animals
Liquid chromatography
Lysis
Magnoliidae
Materia medica, Vegetable
Metabolites
Methanol
Mice
Nonsteroidal anti-inflammatory drugs
Pain
Pain - drug therapy
Pain perception
Plant extracts
Plant Extracts - chemistry
Stems
Toxicity
Uvaria
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cited_by cdi_FETCH-LOGICAL-c476t-aafc6c4f726d5d7a63594f81e936b630aa8530dc5deff3b6b661a8b42b85cea53
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container_title BioMed research international
container_volume 2023
creator Simo, Marguerite Kamdem
Siwe, Gael Tchokomeni
Taboula Kayo, Maurice
Chen, Zheng
Mangoua Kouamo, Mersimine
Dize, Darline
Jazet, Pierre Dongmo
Sameza, Modeste Lambert
Fekam, Fabrice Boyom
Froldi, Guglielmina
description The present study was carried out to investigate the anti-inflammatory activity of a methanolic extract and fractions of Uvaria comperei stems. The crude extract was obtained by maceration of the powder in methanol and fractions by vacuum chromatography from the methanolic extract. To study the anti-inflammatory activity in vitro, red blood cell lysis inhibition assay and albumin denaturation inhibition were performed, while in vivo measurements of carrageenan-induced paw oedema and formalin-induced pain in albino mice were performed. Acute toxicity and cytotoxicity studies of the fraction F2 were performed, as well as its HPLC, and some biochemical parameters were quantified. Uvaria comperei crude extract (UCCE) at 250 and 500 μg/mL completely inhibited albumin denaturation, while decreasing 75.5% of heat blood cell lysis at 500 μg/mL. The fractions 128-136 (F3), 10-11 (F1), and 56-62 (F2) at 500 μg/mL displayed a significant anti-inflammatory activity with percentages of inhibition of 60.5, 67.4, and 100%, respectively. Administration of fraction F2 (25, 50, and 100 mg/kg, p.o.) produced a dose-dependent inhibition of formalin-induced pain of 60.2% at 50 mg/kg in the neurogenic phase (p
doi_str_mv 10.1155/2023/2754725
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The crude extract was obtained by maceration of the powder in methanol and fractions by vacuum chromatography from the methanolic extract. To study the anti-inflammatory activity in vitro, red blood cell lysis inhibition assay and albumin denaturation inhibition were performed, while in vivo measurements of carrageenan-induced paw oedema and formalin-induced pain in albino mice were performed. Acute toxicity and cytotoxicity studies of the fraction F2 were performed, as well as its HPLC, and some biochemical parameters were quantified. Uvaria comperei crude extract (UCCE) at 250 and 500 μg/mL completely inhibited albumin denaturation, while decreasing 75.5% of heat blood cell lysis at 500 μg/mL. The fractions 128-136 (F3), 10-11 (F1), and 56-62 (F2) at 500 μg/mL displayed a significant anti-inflammatory activity with percentages of inhibition of 60.5, 67.4, and 100%, respectively. Administration of fraction F2 (25, 50, and 100 mg/kg, p.o.) produced a dose-dependent inhibition of formalin-induced pain of 60.2% at 50 mg/kg in the neurogenic phase (p&lt;0.05) and 70.2% at 25 mg/kg in the inflammatory phase (p&lt;0.05). Similarly, the time-dependent increase in carrageenan-induced paw circumference induced by carrageenan was inhibited by pretreatment with F2: 50% of inhibition at 25 mg/kg after 30 min (p&lt;0.05) and 96.5% inhibition at 25 mg/kg after 6 h (p&lt;0.05). In this research, the fraction F2 presented its maximum analgesic property at 50 mg/kg, while it presented the highest anti-inflammatory property at 25 mg/kg. The oral lethal median dose (LD50) of F2 was determined to be greater than 2000 mg/kg; further low cytotoxicity in RAW cells was also observed. Overall, this work shows that the methanolic crude extract and fractions, mainly F2, of Uvaria comperei stem have interesting anti-inflammatory properties.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2023/2754725</identifier><identifier>PMID: 36726837</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Acute toxicity ; Albumin ; Albumins ; Analgesics ; Animals ; Anti-inflammatory agents ; Anti-Inflammatory Agents - therapeutic use ; Anti-inflammatory drugs ; Biocompatibility ; Blood ; Carrageenan ; Carrageenan - adverse effects ; Carrageenans ; Chemical properties ; Chemical research ; Cytotoxicity ; Denaturation ; Edema ; Edema - chemically induced ; Edema - drug therapy ; Enzymes ; Erythrocytes ; Flavonoids ; Formaldehyde ; Health aspects ; High performance liquid chromatography ; Inflammation ; Inhibition ; Laboratory animals ; Liquid chromatography ; Lysis ; Magnoliidae ; Materia medica, Vegetable ; Metabolites ; Methanol ; Mice ; Nonsteroidal anti-inflammatory drugs ; Pain ; Pain - drug therapy ; Pain perception ; Plant extracts ; Plant Extracts - chemistry ; Stems ; Toxicity ; Uvaria</subject><ispartof>BioMed research international, 2023, Vol.2023 (1), p.2754725-2754725</ispartof><rights>Copyright © 2023 Marguerite Kamdem Simo et al.</rights><rights>COPYRIGHT 2023 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2023 Marguerite Kamdem Simo et al. 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The crude extract was obtained by maceration of the powder in methanol and fractions by vacuum chromatography from the methanolic extract. To study the anti-inflammatory activity in vitro, red blood cell lysis inhibition assay and albumin denaturation inhibition were performed, while in vivo measurements of carrageenan-induced paw oedema and formalin-induced pain in albino mice were performed. Acute toxicity and cytotoxicity studies of the fraction F2 were performed, as well as its HPLC, and some biochemical parameters were quantified. Uvaria comperei crude extract (UCCE) at 250 and 500 μg/mL completely inhibited albumin denaturation, while decreasing 75.5% of heat blood cell lysis at 500 μg/mL. The fractions 128-136 (F3), 10-11 (F1), and 56-62 (F2) at 500 μg/mL displayed a significant anti-inflammatory activity with percentages of inhibition of 60.5, 67.4, and 100%, respectively. Administration of fraction F2 (25, 50, and 100 mg/kg, p.o.) produced a dose-dependent inhibition of formalin-induced pain of 60.2% at 50 mg/kg in the neurogenic phase (p&lt;0.05) and 70.2% at 25 mg/kg in the inflammatory phase (p&lt;0.05). Similarly, the time-dependent increase in carrageenan-induced paw circumference induced by carrageenan was inhibited by pretreatment with F2: 50% of inhibition at 25 mg/kg after 30 min (p&lt;0.05) and 96.5% inhibition at 25 mg/kg after 6 h (p&lt;0.05). In this research, the fraction F2 presented its maximum analgesic property at 50 mg/kg, while it presented the highest anti-inflammatory property at 25 mg/kg. The oral lethal median dose (LD50) of F2 was determined to be greater than 2000 mg/kg; further low cytotoxicity in RAW cells was also observed. 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Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simo, Marguerite Kamdem</au><au>Siwe, Gael Tchokomeni</au><au>Taboula Kayo, Maurice</au><au>Chen, Zheng</au><au>Mangoua Kouamo, Mersimine</au><au>Dize, Darline</au><au>Jazet, Pierre Dongmo</au><au>Sameza, Modeste Lambert</au><au>Fekam, Fabrice Boyom</au><au>Froldi, Guglielmina</au><au>Muddassir Ali, Muhammad</au><au>Muhammad Muddassir Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-inflammatory, Antinociceptive, and Toxicological Properties of Uvaria comperei Stem Crude Extract and Fractions</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2023</date><risdate>2023</risdate><volume>2023</volume><issue>1</issue><spage>2754725</spage><epage>2754725</epage><pages>2754725-2754725</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>The present study was carried out to investigate the anti-inflammatory activity of a methanolic extract and fractions of Uvaria comperei stems. The crude extract was obtained by maceration of the powder in methanol and fractions by vacuum chromatography from the methanolic extract. To study the anti-inflammatory activity in vitro, red blood cell lysis inhibition assay and albumin denaturation inhibition were performed, while in vivo measurements of carrageenan-induced paw oedema and formalin-induced pain in albino mice were performed. Acute toxicity and cytotoxicity studies of the fraction F2 were performed, as well as its HPLC, and some biochemical parameters were quantified. Uvaria comperei crude extract (UCCE) at 250 and 500 μg/mL completely inhibited albumin denaturation, while decreasing 75.5% of heat blood cell lysis at 500 μg/mL. The fractions 128-136 (F3), 10-11 (F1), and 56-62 (F2) at 500 μg/mL displayed a significant anti-inflammatory activity with percentages of inhibition of 60.5, 67.4, and 100%, respectively. Administration of fraction F2 (25, 50, and 100 mg/kg, p.o.) produced a dose-dependent inhibition of formalin-induced pain of 60.2% at 50 mg/kg in the neurogenic phase (p&lt;0.05) and 70.2% at 25 mg/kg in the inflammatory phase (p&lt;0.05). Similarly, the time-dependent increase in carrageenan-induced paw circumference induced by carrageenan was inhibited by pretreatment with F2: 50% of inhibition at 25 mg/kg after 30 min (p&lt;0.05) and 96.5% inhibition at 25 mg/kg after 6 h (p&lt;0.05). In this research, the fraction F2 presented its maximum analgesic property at 50 mg/kg, while it presented the highest anti-inflammatory property at 25 mg/kg. The oral lethal median dose (LD50) of F2 was determined to be greater than 2000 mg/kg; further low cytotoxicity in RAW cells was also observed. Overall, this work shows that the methanolic crude extract and fractions, mainly F2, of Uvaria comperei stem have interesting anti-inflammatory properties.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>36726837</pmid><doi>10.1155/2023/2754725</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-3387-4102</orcidid><orcidid>https://orcid.org/0000-0002-1771-1939</orcidid><orcidid>https://orcid.org/0000-0002-3147-364X</orcidid><orcidid>https://orcid.org/0000-0002-1556-332X</orcidid><orcidid>https://orcid.org/0000-0002-5936-1789</orcidid><orcidid>https://orcid.org/0000-0003-0170-9516</orcidid><oa>free_for_read</oa></addata></record>
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identifier ISSN: 2314-6133
ispartof BioMed research international, 2023, Vol.2023 (1), p.2754725-2754725
issn 2314-6133
2314-6141
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9886488
source Wiley Online Library Open Access; Publicly Available Content (ProQuest)
subjects Acute toxicity
Albumin
Albumins
Analgesics
Animals
Anti-inflammatory agents
Anti-Inflammatory Agents - therapeutic use
Anti-inflammatory drugs
Biocompatibility
Blood
Carrageenan
Carrageenan - adverse effects
Carrageenans
Chemical properties
Chemical research
Cytotoxicity
Denaturation
Edema
Edema - chemically induced
Edema - drug therapy
Enzymes
Erythrocytes
Flavonoids
Formaldehyde
Health aspects
High performance liquid chromatography
Inflammation
Inhibition
Laboratory animals
Liquid chromatography
Lysis
Magnoliidae
Materia medica, Vegetable
Metabolites
Methanol
Mice
Nonsteroidal anti-inflammatory drugs
Pain
Pain - drug therapy
Pain perception
Plant extracts
Plant Extracts - chemistry
Stems
Toxicity
Uvaria
title Anti-inflammatory, Antinociceptive, and Toxicological Properties of Uvaria comperei Stem Crude Extract and Fractions
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