HADHA alleviates hepatic steatosis and oxidative stress in NAFLD via inactivation of the MKK3/MAPK pathway

• Published in: Molecular biology reports 2023-02, Vol.50 (2), p.961-970
• Main Authors: Ding, Jiexia, Wu, Lili, Zhu, Guoxian, Zhu, Jing, Luo, Pingping, Li, Youming
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Biomedical and Life Sciences; Diet, High-Fat - adverse effects; Fatty acids; Fatty Acids, Nonesterified - metabolism; Fatty liver; High fat diet; Histology; Inflammation - metabolism; Life Sciences; Lipid Metabolism; Liver - metabolism; Liver diseases; MAP kinase; Metabolic syndrome; Mice; Mice, Inbred C57BL; Mitochondria; Mitochondrial Trifunctional Protein, alpha Subunit - metabolism; Morphology; Non-alcoholic Fatty Liver Disease - pathology; Original; Original Article; Oxidative metabolism; Oxidative Stress; Signal transduction; Steatosis
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-022-07965-2

Background Nonalcoholic fatty liver disease (NAFLD) is a liver metabolic syndrome and still lacks effective treatments because the molecular mechanism underlying the development of NAFLD is not completely understood. We investigated the role of Hydroxyl CoA dehydrogenase alpha subunit (HADHA) in the pathogenesis of NAFLD. Methods HADHA expression was detected both in NAFLD cell and mice, and knockdown of HADHA in free fatty acids (FFA)-treated L02 or overexpression of HADHA in high fat diet (HFD)-fed mice was used to detected the influence of HADHA on hepatic steatosis, mitochondrial dysfunction, and oxidative stress by regulating of MKK3/MAPK signaling. Results Our data revealed that HADHA expression was decreased in FFA-treated L02 cells and in HFD-fed mice. Knockdown of HADHA markedly aggravated hepatic steatosis, inflammation and oxidative stress in FFA-treated L02 cells, which was associated with the activation of MKK3/MAPK signalling pathways. Moreover, oxidative stress and liver lesions were improved in NAFLD mice by upregulation of HADHA. Importantly, we demonstrated that overexpression of HADHA inhibited the expression of p-MAPK in NAFLD mice, reducing lipid accumulation and steatosis. Conclusion HADHA may function as a protective factor in the progression of NAFLD by alleviating abnormal metabolism and oxidative stress by suppressing MKK3/MAPK signalling pathway activation, providing a new target for the treatment of NAFLD.