Effect of ethanol extract from Chrysanthemum cinerariifolium leaves on Ki-67 proliferation and dysplasia severity in a rat model of oral squamous cell carcinoma

• Published in: Open veterinary journal (Tripoli, Libya) Libya), 2023-01, Vol.13 (1), p.99-107
• Main Authors: Listiyana, Anik, Kristanti, Risma Aprinda, Aishaqeena, Al Mazida Fauzil, Ahmad, Anggun Putri Maulana, Astari, Lina Fitria, Indradmojo, Christyaji, Inayatilah, Fidia Rizkiah
• Format: Article
• Language: English
• Subjects: Animals; Cell Proliferation; Chrysanthemum cinerariifolium - chemistry; Disease Models, Animal; Ki-67 Antigen; Male; Mouth Neoplasms - chemically induced; Mouth Neoplasms - drug therapy; Original Research; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; Squamous Cell Carcinoma of Head and Neck - chemically induced; Squamous Cell Carcinoma of Head and Neck - drug therapy
• ISSN: 2226-4485; 2218-6050
• DOI: 10.5455/OVJ.2023.v13.i1.10

Oral squamous cell carcinoma (OSCC) is a malignant tumor that can rapidly infiltrate the oral epithelial tissue and cause high mortality worldwide because the available therapies are less effective. leaf contains secondary metabolites as anti-inflammatory, antioxidant, anticancer, and antimutagenic. The study aimed to analyze the ethanolic extract of leaf in reducing proliferation (Ki-67) and the degree of dysplasia in OSCC rats. This study used male Sprague Dawley induced by 7,12-dimethylbenz(a)anthracene (DMBA) 0.5% and divided into five treatment groups, namely positive control/C+ (sick), negative control/C- (healthy), and treatment group induced with DMBA and given extract leaf with successive doses of T1, T2, and T3 (50, 100, and 200 mg/kg bw). The oral epithelium was stained with hematoxylin and eosin and immunohistochemically stained with a Ki-67 monoclonal antibody. The statistical analysis utilizes the one-way analysis of variance test. The results showed that T1 at a dose of 200 mg/kg bw could significantly reduce Ki-67 expression and the degree of oral epithelial dysplasia (OED; < 0.05) close to healthy controls. The conclusion shows that leaf extract can be a therapy against OSCC by decreasing cell proliferation and the degree of OED.