Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice

Zinc (Zn) is an essential cofactor for physiological homeostasis in the body. Zn oxide (ZnO), an inorganic compound that supplies Zn, exists in various sizes, and its bioavailability may vary depending on the size . However, comparative studies on the nutritional effects of micro-sized ZnO (M-ZnO) a...

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Bibliographic Details
Published in:Journal of veterinary science (Suwŏn-si, Korea) Korea), 2023, Vol.24 (1), p.15-27
Main Authors: Yoon, Ja-Seon, Nam, Sang Yoon, Lee, Beom Jun, Lee, Hyun Jik
Format: Article
Language:English
Subjects:
Animals
Male
Mice
Original
RNA, Messenger
Zinc - therapeutic use
Zinc Oxide - pharmacology
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Summary:Zinc (Zn) is an essential cofactor for physiological homeostasis in the body. Zn oxide (ZnO), an inorganic compound that supplies Zn, exists in various sizes, and its bioavailability may vary depending on the size . However, comparative studies on the nutritional effects of micro-sized ZnO (M-ZnO) and nano-sized ZnO (N-ZnO) supplementation on Zn deficiency (ZnD) animal models have not been reported. This study investigated the nutritional bioavailability of N-ZnO and M-ZnO particles in dietary-induced ZnD mice. Animals were divided into six experimental groups: normal group, ZnD control group, and four ZnO treatment groups (Nano-Low, Nano-High, Micro-Low, and Micro-High). After ZnD induction, N-ZnO or M-ZnO was administered orally every day for 4 weeks. ZnD-associated clinical signs almost disappeared 7 days after N-ZnO or M-ZnO administration. Serum Zn concentrations were higher in the Nano-High group than in the ZnD and M-ZnO groups on day 7 of ZnO treatment. In the liver and testis, Nano-Low and Nano-High groups showed significantly higher Zn concentrations than the other groups after 14-day treatment. ZnO supplementation increased mRNA expression in the liver and testis and mRNA expression in the liver. Based on hematoxylin-and-eosin staining results, N-ZnO supplementation alleviated histological damage induced by ZnD in the testis and liver. This study suggested that N-ZnO can be utilized faster than M-ZnO for nutritional restoration at the early stage of ZnD condition and presented as an indicator of Zn status in the serum, liver, and testis.
ISSN:1229-845X
1976-555X
DOI:10.4142/jvs.22201